CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2022 , Vol. 40 >Issue 2: 236 - 241

DOI: https://doi.org/10.12140/j.issn.1000-7423.2022.02.016

SHORT COMMUNICATIONS

Affect of Trichomonas vaginalis excretory secretory protein on sperm quality of mice

  • LUO Cheng-yang ,
  • HAO Li-xia ,
  • GUO Li-hua ,
  • WANG Bing-li ,
  • JI Si-fan ,
  • LIAN An-na ,
  • ZHU Xiao-hui ,
  • MEI Xue-fang ,
  • TIAN Xiao-wei ,
  • WANG Shuai ,
  • ZHANG Zhen-chao
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  • 1. School of Basic Medicine, Xinxiang Medical College, Xinxiang 453003, China
    2. Key Laboratory of Pathogen Biology, Xinxiang Medical College, Xinxiang 453003, China
    3. Xinxiang Maternal and Child Health Hospital, Xinxiang 453003, China

Received date: 2021-07-30

  Revised date: 2021-11-19

  Online published: 2022-04-13

Supported by

National Natural Science Foundation of China(81802028);Science and Technology Project of Henan Province(212102310716);Innovation and entrepreneurship training program for College Students of Henan Province(S202110472024);Science and Technology Project of Xinxiang City(GG2019034);Science and Technology Project of Xinxiang City(GG2021011)

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Abstract

Trichomonas vaginalis excretory secretory protein (TvESP) was collected for determining the concentration using Bradford method kit and further analyzed of TvESP by thesodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Mouse sperm were collected from the epididymis of Kunming mice, and the sperm density was adjusted to 1 × 107/ml. Sperm were divided into 6 groups and co-cultured with TvESP at final concentrations of 0, 5, 10, 20, 40 and 80 ng/μl, respectively. After co-culturing with TvESP for 1 h, sperm motility was observed using an inverted microscope, and sperm apoptosis was detected by flow cytometry. After co-culturing for 0.5, 1 and 2 h, the sperm viability was analyzed by trypan blue staining. After sperm were incubated with TvESP at final concentrations of 0 and 40 ng/μl for 1 h, sperm were stained with a fluorescent dye and the acrosome was observed under a fluorescence microscope. One-way ANOVA was used for comparing between groups. The results showed that the percentage of active sperm in the 5, 10, 20, 40 and 80 ng/μl groups were (31.90 ± 4.10)%, (23.00 ± 4.90)%, (18.80 ± 3.50)%, (13.60 ± 1.20)% and (8.60 ± 1.20)%, respectively, which were significantly lower than that of the 0 ng/μl concentration group (44.50 ± 3.20)% (F = 30.76, P < 0.01). The apoptosis rates of sperm in the 5, 10, 20, 40 and 80 ng/μl groups were (17.99 ± 1.73)%, (22.43 ± 1.53)%, (26.76 ± 2.20)%, (32.05 ± 1.68)% and (41.37 ± 2.28)%, respectively, which were significantly higher than that of the 0 ng/μl group (15.42 ± 1.10)% (F = 256.79, P < 0.01). After treatment with TvESP for 0.5 h, the mortality rates of sperm treated with 10 ng/μl (11.90 ± 1.70)%, 20 ng/μl (14.10 ± 1.40)%, 40 ng/μl (16.30 ± 1.20)%, 80 ng/μl (17.90 ± 1.30)% of TvESP were significantly higher than that of the 0 ng/μl group (7.80 ± 1.10)% (F = 15.72, P < 0.05). The sperm mortality rate in the 5 ng/μl group (10.10 ± 1.40)% was not statistically different from that of the 0 ng/μl group. After treatment with TvESP for 1 h, the sperm mortality rates in the 5, 10, 20, 40 and 80 ng/μl groups were (12.90 ± 0.90)%, (14.60 ± 0.90)%, (16.20 ± 1.30)%, (18.10 ± 1.10)%, (20.00 ± 1.40)%, which were significantly higher than that of the 0 ng/μl group (9.90 ± 1.90)% (F = 15.76, P < 0.01). After 2 h treatment, the mortality rates in the 10, 20, 40 and 80 ng/μl groups were (17.00 ± 1.20)%, (18.30 ± 1.20)%, (20.50 ± 1.20)% and (22.80 ± 1.50)%, respectively, which were significantly higher than that of the 0 ng/μl group (12.90 ± 0.50)% (F = 22.22, P < 0.01), and the mortality rate in the 5 ng/μl group (15.00 ± 0.40)% was not statistically different from that of the 0 ng/μl group. After co-culturing the sperm with TvESP at a final concentration of 40 ng/μl for 1 h, the integrity of acrosome was destroyed. All results suggested that TvESP can reduce sperm quality.

Key words： Trichomonas vaginalis; Secretory protein; Mouse; Sperm quality

Cite this article

LUO Cheng-yang , HAO Li-xia , GUO Li-hua , WANG Bing-li , JI Si-fan , LIAN An-na , ZHU Xiao-hui , MEI Xue-fang , TIAN Xiao-wei , WANG Shuai , ZHANG Zhen-chao . Affect of Trichomonas vaginalis excretory secretory protein on sperm quality of mice[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(2) : 236 -241 . DOI: 10.12140/j.issn.1000-7423.2022.02.016

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