CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2022 , Vol. 40 >Issue 2: 181 - 186

DOI: https://doi.org/10.12140/j.issn.1000-7423.2022.02.008

ORIGINAL ARTICLES

Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang

  • CAI Ren ,
  • REN Yuan ,
  • MI Rong-sheng ,
  • GUO Gang ,
  • QI Wen-jing ,
  • ZHANG Zhuang-zhi ,
  • GUO Bao-ping
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  • 1. Institute of Clinical Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    2. State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    3. Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
    4. Veterinary Research Institute, Xinjiang Academy of Animal Sciences (Animal Clinical Medical Research Center of Veterinary Research Institute, Xinjiang), Urumqi 830000, China

Received date: 2021-08-12

  Revised date: 2021-10-01

  Online published: 2022-04-07

Supported by

Natural Science Foundation of Xinjiang Uygur Autonomous Region(2019D01C315)

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Abstract

Objective To understand the characteristics of alveolar echinococcosis (AE) cases and the genetic polymorphism of the parasites in Xinyuan County, Xinjiang, to provide reference information for formulating developing prevention and control strategies for AE. Methods During 2016—2020, information on AE patients and some surgically resected liver lesion samples from Xinyuan County in the biological sample bank of the First Affiliated Hospital of Xinjiang Medical University were collected. Genomic DNA of the liver lesion samples was extracted, and the mitochondrial NADH dehydrogenase 2 (nad2) gene was amplified by PCR and sequenced. The PCR products were sequenced and spliced for Blast comparison. Mega10.0 software was used to construct a phylogenetic tree by the neighbor-joining method, DnaSPv5 software was used to analyze the genotype of amplified sequence, and Network10.0 software was used to make nad2 genotype network map. Results A total of 28 AE cases were collected, including 7 in rural areas and 21 in Kunes River Basin, among which 60.71% (17/28) were male, and 39.29% (11/28) were female (χ2 = 0.704, P < 0.05). Ethnic distribution was mainly Han (42.86%, 12/28) and Kazak (39.29%, 11/28), and the highest infection rate among Mongolians was 11.17%. There was a significant difference in the infection rate among different ethnic groups (χ2 = 61.08, P < 0.01). Farmers and herdsmen were the dominant occupations, accounting for 92.86% (26/28), while only 7.14% (2/28) were of other occupations. There was no significant difference in infection rate among different age groups (χ2 = 0.418, P > 0.05). A expected, 25 samples were amplified by PCR with a fragment size of 1 031 bp. Sequence alignment results showed that there were four genotypes: H1, H2, H3 and H4, among which H1 was the main genotype and the sequence consistency was 100% with Kazakhstan (GenBank accession no. AB461406). Phylogenetic tree results showed that the four genotypes in Xinyuan county isolates were in the same branch as the Poland (GenBank accession no. KY205700) and Kazakhstan (GenBank accession no. AB461406), but more distantly related to France (GenBank Accession no. AB461404), Austria (GenBank accession no. AB461403), Canada (GenBank accession no. JF751036), Alaska (GenBank accession no MT429275), Indiana, USA (GenBank accession no. AB461409) and Inner Mongolia, China (GenBank accession no. AB461411). According to the results of centering on the H1, the genotype network diagram showed H2, H3, H4, Sichuan of China, Poland, Canada and Japan were scattered distributed which indicated that the isolates with H1 has a very close relationship, but it is not in the same clade with France, Austria, Canada, Alaska, USA isolates, and had a distant relationship. Conclusion The majority of AE patients were Han and Kazakian farmers and herdsmen, and the Mongolians had the highest infection rate. There were 4 genotypes in AE patients, among which H1 was the main genotype in Xinyuan County.

Key words： Alveolar echinococcoisis; Xinyuan County; Nad2 gene; Haplotype

Cite this article

CAI Ren , REN Yuan , MI Rong-sheng , GUO Gang , QI Wen-jing , ZHANG Zhuang-zhi , GUO Bao-ping . Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(2) : 181 -186 . DOI: 10.12140/j.issn.1000-7423.2022.02.008

References

[1] Deplazes P,, Rinaldi L,, Alvarez Rojas CA, et al. Global distribution of alveolar and cystic echinococcosis[J]. Adv Parasitol, 2017, 95: 315-493.
[2] Nahorski WL,, Knap JP,, Pawlowski ZS, et al. Human alveolar echinococcosis in Poland: 1990-2011[J]. PLoS Negl Trop Dis, 2013, 7(1): e1986.
[3] Li LH,, Wang W,, Hou XL, et al. Affects of Echinococcus multilocularis metacestode infection on the natural killer T cells and their subsets in mouse spleen[J]. Chin J Parasitol Parasit Dis, 2021, 39(3): 311-317. (in Chinese)
[3] (李玲慧,, 王慧,, 侯昕伶, 等. 多房棘球蚴感染对小鼠脾自然杀伤T细胞及其亚群的影响[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 311-317.)
[4] Guo BP. Study on correlation between pathogenic differences and mitochondrial genetic markers in Echinococcus multilocularis[D]. Shihezi: Shihezi University, 2019: 1-3. (in Chinese)
[4] (郭宝平. 多房棘球绦虫致病差异与线粒体遗传标志相关性的研究[D]. 石河子:石河子大学, 2019: 1-3.)
[5] Li WD,, Wen H,, Hou J, et al. Role of extracellular matrix protein 1 in the liver fibrosis induced by Echinococcus multilocularis infection in mice[J]. Chin J Parasitol Parasit Dis, 2021, 39(3): 296-303. (in Chinese)
[5] (李文定,, 温浩,, 侯娇, 等. 细胞外基质蛋白1在多房棘球蚴感染小鼠肝纤维化过程中的作用[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 296-303.)
[6] Wen H,, Vuitton L,, Tuxun T, et al. Echinococcosis: advances in the 21st century[J]. Clin Microbiol Rev, 2019, 32(2): e00075-18.
[7] Zhang C,, Shao Y,, Yang S, et al. T-cell tolerance and exhaustion in the clearance of Echinococcus multilocularis: role of inoculum size in a quantitative hepatic experimental model[J]. Sci Rep, 2017, 7(1): 3424.
[8] Guo B,, Zhang Z,, Guo Y, et al. High endemicity of alveolar echinococcosis in Yili Prefecture, Xinjiang Autonomous Region, the People’s Republic of China: infection status in different ethnic communities and in small mammals[J]. PLoS Negl Trop Dis, 2021, 15(1): e0008891.
[9] Nakao M,, Yokoyama N,, Sako Y, et al. The complete mitochondrial DNA sequence of the cestode Echinococcus multilocularis (Cyclophyllidea ∶ Taeniidae)[J]. Mitochondrion, 2002, 1(6): 497-509.
[10] Wei YH,, Liu H,, Li WJ, et al. Analysis nad1 gene polymorphism of Echinococcus granulosus isolates from humans in Ali region of Tibet[J]. Chin J Parasitol Parasit Dis, 2020, 38(1): 17-21. (in Chinese)
[10] (魏玉环,, 刘华,, 李武军, 等. 西藏阿里地区细粒棘球蚴人体分离株nad1基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1): 17-21.)
[11] Vanhove MPM,, Briscoe AG,, Jorissen MWP, et al. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes ∶ Gyrodactylidae and Dactylogyridae)[J]. BMC Genomics, 2018, 19(1): 520.
[12] Guo B,, Zhang Z,, Zheng X, et al. Prevalence and molecular characterization of Echinococcus granulosus sensu stricto in northern Xinjiang, China[J]. Korean J Parasitol, 2019, 57(2): 153-159.
[13] Staubach C,, Thulke HH,, Tackmann K, et al. Geographic information system-aided analysis of factors associated with the spatial distribution of Echinococcus multilocularis infections of foxes[J]. Am J Trop Med Hyg, 2001, 65(6): 943-948.
[14] Israyin U,, Tong SX,, Meng H, et al. Epidemiological survey of echinococcosis in Xinjiang in 2003[J]. Endem Dis Bull, 2009, 24(1): 65-67. (in Chinese)
[14] (伊斯拉音·乌斯曼,, 童苏祥,, 孟贺巴特, 等. 2003年新疆包虫病流行病学调查报告[J]. 疾病预防控制通报, 2009, 24(1): 65-67.)
[15] Maimaitijiang W,, Adili S,, Yisilayin O, et al. Epidemiological survey of echinococcosis in Xinjiang Uygur Autonomous Region in 2012[J]. Chin J Parasitol Parasit Dis, 2016, 34(3): 249-254. (in Chinese)
[15] (买买提江·吾买尔,, 阿迪力·司马义,, 伊斯拉音·乌斯曼, 等. 2012年新疆维吾尔自治区人群棘球蚴病流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(3): 249-254.)
[16] Gao YS,, Zhu MB,, Guo YZ, et al. Clinical analysis on hepatic hydatid disease in Yili River Valley[J]. Chin J Parasitol Parasit Dis, 2016, 23(1): 3-13. (in Chinese)
[16] (高永盛,, 朱马拜,, 郭永忠, 等. 新疆伊犁河谷肝棘球蚴病临床资料分析[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(1): 3-13.)
[17] Karamon J,, Stojecki K,, Samorek-Pierog M, et al. Genetic diversity of Echinococcus multilocularis in red foxes in Poland: the first report of a haplotype of probable Asian origin[J]. Folia Parasitol (Praha), 2017, 9: 64.
[18] Nakao M,, Xiao N,, Okamoto M, et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis[J]. Parasitol Int, 2009, 58(4): 384-389.
[19] Otero-Abad B,, Torgerson PR. A systematic review of the epidemiology of echinococcosis in domestic and wild animals[J]. PLoS Negl Trop Dis, 2013, 7(6): e2249.
[20] Wu C,, Zhang W,, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China[J]. Parasit Vectors, 2017, 10(1): 265.
[21] Jia WZ,, Yan HB,, Guo AJ, et al. Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance[J]. BMC Genomics, 2010, 11: 447.
[22] Torgerson PR,, Keller K,, Magnotta M, et al. The global burden of alveolar echinococcosis[J]. PLoS Negl Trop Dis, 2010, 4(6): e722.
[23] Torgerson PR,, Schweiger A,, Deplazes P, et al. Alveolar echinococcosis: from a deadly disease to a well-controlled infection. Relative survival and economic analysis in Switzerland over the last 35 years[J]. J Hepatol, 2008, 49(1): 72-77.
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