CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2022 , Vol. 40 >Issue 1: 121 - 123

DOI: https://doi.org/10.12140/j.issn.1000-7423.2022.01.019

SHORT COMMUNICATIONS

Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients

  • Jia LIU ,
  • Jun-ying MA ,
  • Xue-fei ZHANG ,
  • Hu WANG ,
  • Xiao MA ,
  • Hui-xia CAI ,
  • Qi GAO ,
  • Shuang QIE ,
  • Yan YIN ,
  • Wei WANG ,
  • Yong-shun WANG ,
  • Jing-xiao ZHANG ,
  • Yu-fang LIU ,
  • Wen LEI ,
  • Pei-zhen ZHAN ,
  • Qing ZHANG
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  • 1 Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
    2 Health Commission of Qinghai Province, Xining 810000, China
    3 Beijing Hotgen Biotechnology Co., Ltd, Beijing 100000, China

Received date: 2021-07-27

  Revised date: 2021-11-16

  Online published: 2022-01-30

Supported by

2020 Science and Technology Plan Project of Qinghai Province(2020-SF-133)

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Abstract

To evaluate the effect of up-converting phosphor-lateral flow (UPT-LF) method for rapid detection of IgG antibodies in echinococcosis patient, and compare the test results with that of enzyme-linked immunosorbent assay (ELISA) method. Serum samples from patients with clinically confirmed echinococcosis were collected at Qinghai Provincial People’s Hospital and the Affiliated Hospital of Qinghai University from 2017 to 2018. Serum samples of healthy individuals who came for general healthy checks were also collected from the physical examination centers of those hospitals. The anti-echinococcosis IgG antibody in the samples was detected by UPT-LF and ELISA methods. The sensitivity, specificity, misdiagnosis rate, total coincidence rate and Yoden index were analyzed based on clinical diagnosis results. Chi-square test was performed to compare the results between the two methods. A total of 104 serum samples of echinococcosis patients, including 46 cases of alveolaris echinococcosis (AE), 58 cases of cyst echinococcosis (CE) and 165 serum samples from healthy individuals were collected. The sensitivity of UPT-LF and ELISA in echinococcosis patients were 94.2% (98/104) and 78.8% (82/104), respectively, with statistically significant difference between the two methods (χ2 = 11.250, P < 0.01). The sensitivity of UPT-LF and ELISA in AE patients were 97.8% (45/46) and 89.1% (41/46), while 91.4% (53/58) and 70.7% (41/58) in CE patients, respectively. The specificity of UPT-LF and ELISA in non-echinococcosis patients were 95.2% (157/165) and 90.9% (150/165), respectively, with no statistically significant difference between the two methods (χ 2 = 1.565, P > 0.05). Compared with the clinical diagnosis result, the coincidence rates of UPT-LF and ELISA were 94.8% (255/269) and 86.2% (232/269), respectively, with no statistically significant difference between the two methods (χ 2 = 1.884, P > 0.05). The Youden index of UPT-LF and ELISA were 0.894 and 0.697, respectively. UPT-LF method showed higher sensitivity than that of ELISA, and has a good application prospect in the field for the rapid detection of echinococcosis.

Key words： Echinococcosis; Up-converting phosphor-lateral flow; Rapid detection

Cite this article

Jia LIU , Jun-ying MA , Xue-fei ZHANG , Hu WANG , Xiao MA , Hui-xia CAI , Qi GAO , Shuang QIE , Yan YIN , Wei WANG , Yong-shun WANG , Jing-xiao ZHANG , Yu-fang LIU , Wen LEI , Pei-zhen ZHAN , Qing ZHANG . Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022 , 40(1) : 121 -123 . DOI: 10.12140/j.issn.1000-7423.2022.01.019

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