CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >
Investigation on common infection of diarrhea-causing parasitic protozoa in Shanghai during 2015—2019
Received date: 2021-04-28
Revised date: 2021-06-24
Online published: 2021-12-17
Supported by
Shanghai Municipal Health Commission Scientific Research Project(201940099);Fifth Round of Three-Year Public Health Action Plan of Shanghai(GWV-10.1-XK13)
To understand common infection status of diarrhea-causing parasitic protozoa in residents of Shanghai Municipality, a survey was carried out through selecting 1-2 general hospitals from five districts, including Changning, Hongkou, Minhang, Jiading and Pudong New District, as surveillance sites in 2015—2019. Fecal samples were collected from diarrhea patients (defecation for more than three times within 24 h, with abnormal feces appearance), who visited the surveillance site hospital, based on the principle of sampling from both urban and the suburb districts. Using the methods of direct smear with saline, iodine staining, acid-fast staining and coproantigen immuno-chromatography, the fecal samples were examined for Entamoeba histolytica, Giardia lamblia and Cryptosporidium, and analyzing the infection status. The results showed that a total of 6 984 fecal samples were collected from diarrhea patients and examined etiologically, of them 9 were found positive, with an overall infection rate of 0.13% (9/6 984) in 2015—2019. Among the positives, 4 were infected with E. histolytica, 2 were G. lamblia and 3 were Cryptosporidium. The positive rates in each year were 0.08% (1/1 207), 0.40% (5/1 254), 0.13% (2/1 506), 0 (0/1 509) and 0.07% (1/1 508), respectively. Among the 6 984 patients with diarrhea, the age group of 30-40 years (1 370) had 4 cases of protozoa infection; of 3 345 males, 4 were found protozoa positive, while from 3 639 females, 5 were positive; there were 2, 2, 2, 3 positive cases were detected in districts of Changning, Hongkou, Minhang, and Jiading, whereas no positive cases were detected in Pudong New District. Protozoan coproantigen fast test strip detected 31 positives, with an overall positive rate of 0.44% (31/6 984), which was significantly different from the findings by pathogen examination (χ2 = 12.13,P < 0.01). The positive rate of parasitic protozoa was found at a considerably low level, maintaining at low prevalence and in scattering distribution in Shanghai. Therefore, it is urged to carry on health education, and to strengthen continuous and effective surveillance; applying combined approaches of etiology, immunology, and molecular biology for improving the detection rate.
Key words： Diarrhea; Parasitic protozoon; Infection; Shanghai City
Zhen-yu WANG , Yao-guang ZHANG , Xiao-jiang MA , Li JIANG , Yan-yan HE , Qian ZHU , Hao PAN . Investigation on common infection of diarrhea-causing parasitic protozoa in Shanghai during 2015—2019[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021 , 39(6) : 759 -763 . DOI: 10.12140/j.issn.1000-7423.2021.06.020
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