In accordance with the Implementation Plan for Surveillance and Investigation of Key Human Parasitic Diseases in Guangxi, a survey on human soil-borne nematodiasis (including hookworm disease, ascariasis, whipworm disease, pinworm disease) and clonorchiasis was performed in Baise City, Guanxi, from 2016 to 2019. Stool samples were collected from 200 residents over 3 years old at each site, and examined with modified Kato-Katz thick smear method for the eggs of soil-borne nematodes and Clonorchis sinensis and counted. Hookworm species were identified using larva test-tube filter paper culture method for Ancylostoma duodenale and Ancylostoma americana; Enterobius eggs were examined with cellophane anal swab for children aged 3-12 years. The survey of human parasitic diseases were carried out for 14 524 person-times in the city in 2016—2019. The results showed that a total of 1 464 persons were found infected, with a infection rate of 10.08%. The infection rate was highest in 2018 (16.72%, 508/3 039), and lowest in 2016 (6.79%, 283/4 168). The infection rate was highest ofC. sinensis (4.96%, 721/14 524) followed by hookworms (3.48%, 80/14 524). The infection rate was 6.94% (452/14 524) in males and 12.63% (1 012/14 524) in females (P < 0.01). The infection rate was high in populations aged 13-20 years, residing in rural areas, with an occupation of farmers, and having an education level of illiteracy, and in Tianyang District, which were 21.46% (362/1 268), 29.58% (1 039/3 512), 13.39% (641/4 787), 19.96% (641/3 211) and 13.50% (561/4 155), respectively. A total of 2 485 children were examined during the 4 years, of them 166 were infected with Enterobius vermicularis (6.68%, 166), and the highest rate was found in 7-year-old children (10.45%, 23/220). The infection rate was 5.83% (69/1 184) in boys and 7.46% (97/1 301) in girls. The results indicate that the infection rate of human parasites in Baise showed a trend of increase followed by a decrease during 2016—2019. The infection rate ofC. sinensis was relatively high, but the infection of soil-borne nematode was predominated by hookworm infection. It is necessary to strengthen the treatment, and health education in the key populations aged 13-20 years, residing in rural areas, with an occupation of farmers, and having an education level of illiteracy, and in Tianyang District.