CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2021 , Vol. 39 >Issue 3: 327 - 332

DOI: https://doi.org/10.12140/j.issn.1000-7423.2021.03.006

ORIGINAL ARTICLES

Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang

  • Bao-ping GUO ,
  • Gang GUO ,
  • Li ZHANG ,
  • Jing-jing XIANG ,
  • Xiao-ping WANG ,
  • Yuan REN ,
  • Wen-jing QI ,
  • Hui ZHANG ,
  • Jun LI ,
  • Wen-bao ZHANG ,
  • Hai-yan WANG
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  • 1 The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    2 Chabuchaer County People’s Hospital, Chabuchaer 835300, China
    3 Chabuchaer Center for Disease Control and Prevention, Chabuchaer 835300, China
    4 Yili Prefecture Center for Disease Control and Prevention, Yining 835000, China
    5 State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
    6 Basic Medical College of Xinjiang Medical University, Urumqi 830011, China

Received date: 2020-12-22

  Revised date: 2021-01-21

  Online published: 2021-07-05

Supported by

National Natural Sciecne Foundation of China(81830066);National Natural Sciecne Foundation of China(U1803282);National Natural Sciecne Foundation of China(31860703)

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Abstract

Objective To investigate the infection and distribution of Echinococcus multilocularis in small rodents, the intermediate hosts, in Chabuchaer County, Xinjiang, and to identify the intermediate host species of the parasite, providing scientific basis for formulating local control measures. Methods Small rodents were captured with plate clamps and bow clips in four selected investigation sites, including Jiagasitai town, Qiongbole town, Sishi and Dulata Port. Suspected infection in liver tissues was confirmed by HE staining and PCR amplification. The PCR products were sequenced. The species of small rodents were identified according to their physical appearance and skull morphology. Theχ2 test was used to compare the infection rate in different regions and in different rodent populations. Results A total of 595 small rodents were captured, which belonged to 7 species, including 464 Microtus spp., 84 Apodemus sylvaticus, 14 Meriones tamariscinus Pallas, 14 Rhombomys sopimus Pallas, 8 Ellobius trancrei, 6 Cricetulus migratorius Pallas and 5 Meriones erythrourus Pallas. In 67 liver samples of suspected infection, lesions of E. multilocularis metacestodes were observed. HE staining showed that the protoscoleces of E. multilocularis metacestodes were visible in the lesion tissues. PCR amplification and sequence alignment showed that all the 67 small rodents were infected with metacestodes of E. multilocularis, with a total infection rate of 11.3%. All the seven species of rodents were infected, with infection rates of 12.8% (57/464), 3.6% (3/84), 1/14, 2/14, 2/8, 1/5 and 1/6 for Microtus spp, A. sylvaticus, M. tamariscinus Pallas, R. sopimus Pallas, E. trancrei, M. serythrourus Pallas and C. migratorius Pallas, respectively. Chi-square analysis showed that there was a significant difference in the infection rate between Microtus spp. and A. sylvaticus (P < 0.05). The infection rate of Microtus spp. did not significantly differ from that of the other species (P > 0.05). Of the 4 investigation sites, the Qiongbole town showed the highest infection rate (12.3%, 49/398). Conclusion The small rodents captured in Chabuchaer County were mainly Microtus spp. and A. sylvaticus, with the former species showing the highest infection rate of metacestodes.

Key words： Metacestodes of Echinococcus multilocularis; Echinococcus multilocularis; Small rodent; Microtus spp.; Chabuchaer County; Xinjiang

Cite this article

Bao-ping GUO , Gang GUO , Li ZHANG , Jing-jing XIANG , Xiao-ping WANG , Yuan REN , Wen-jing QI , Hui ZHANG , Jun LI , Wen-bao ZHANG , Hai-yan WANG . Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021 , 39(3) : 327 -332 . DOI: 10.12140/j.issn.1000-7423.2021.03.006

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