CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >
Analysis on laboratory diagnosis efficacy and reexamination of the results for imported malaria cases in Tai’an City of Shandong Province during 2015—2019
Received date: 2020-07-27
Revised date: 2020-09-14
Online published: 2021-03-10
Supported by
Natural Science Fundation of Shandong Province(ZR2017YL005);Zhifei Disease Prevention and Control Technology Research Fund of Shandong Preventive Medicine Association(LYH2017-16)
The imported malaria cases in Tai’an City, Shandong Province, during 2015—2019 were diagnosed and the results rechecked at Province-, city-, and county-based laboratories using microscopy, nested-PCR, and colloidal gold methods, respectively. A total of 122 imported malaria cases were diagnosed and the results rechecked by the city laboratory. Rechecking the results, the provincial malaria diagnosis reference laboratory confirmed 122 cases positive, among them, the coincidence rate by microscopy, colloidal gold and PCR methods was 94.3% (115/122), 95.9% (117/122), and 100%, respectively; 7 cases of microscopy negative were falciparum malaria; while of 5 cases of colloidal gold method negative, 2 cases were falciparum, and 3 non-falciparum. There was a significant difference in the coincidence rate among the three examination methods (P < 0.05). In addition, there was a significant difference in the coincidence rate of laboratory diagnosis for four plasmodium species (P < 0.01), among them, the diagnosis coincidence rate for Plasmodium falciparum and P. vivax was highest (100%), whereas the lowest coincidence was seen in P. ovale (12/18). The pass rate of diagnosis at the first visit in medical institutions was 94.3% (115/122), and the pass rate of rechecking at city-level and county-level centers for disease control (CDC) were 97.5% (119/122) and 95.1% (116/122), respectively (P > 0.05). It is necessary to further strengthen skill training for laboratory microscopy examiners and actively carry out malaria nucleic acid testing, in order to improve the performance level of diagnosis in medical institutions and CDCs.
Key words： Malaria; Imported cases; Microscopy; Nested-PCR; Colloidal gold method; Accuracy
Xin-feng ZHANG , Yun-qiang LIU , Zhi-jun ZHANG , Yan XU , Jin LI , Ben-guang ZHANG . Analysis on laboratory diagnosis efficacy and reexamination of the results for imported malaria cases in Tai’an City of Shandong Province during 2015—2019[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021 , 39(1) : 129 -132 . DOI: 10.12140/j.issn.1000-7423.2021.01.020
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