CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2020 , Vol. 38 >Issue 6: 792 - 795

DOI: https://doi.org/10.12140/j.issn.1000-7423.2020.06.021

SHORT COMMUNICATIONS

Evaluation of the performance of three immunochromatographic strips in detecting Leishmania infection in dogs in Gansu Province

  • Da-wei YU ,
  • Fan LI ,
  • Yu FENG ,
  • Cheng-ming YANG ,
  • Jun-ke YANG ,
  • Lin-lin LIU ,
  • Yong-fu ZHANG
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  • 1 Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730020, China
    2 Wenxian Center for Disease Control and Prevention, Longnan 746400, China
    3 Diebu Center for Disease Control and Prevention, Gannan Autonomous Prefecture 747400, China

Received date: 2020-06-28

  Online published: 2021-01-12

Supported by

National Natural Science Foundation for Youth(81703171)

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Abstract

Blood samples were collected from 537 domestic dogs in Wenxian County and Diebu County of Gansu Province, where canine visceral leishmaniasis were endemic, and from 37 domestic dogs from Tianzhu County of Gansu Province, the non-endemic area. Blood examination was carried out using three immunochromatographic test strips including human rk39 strip, canine rk39 strip and circulating antigen (CA) strip in parallel to assess the sensitivity and specificity of the methods, and using PCR assay as the standard to compare with. The results showed that among the total 574 dog blood samples, 221 samples were tested Leishmania positive by PCR with the positive rate of 38.5% (221/574). Examining the 221 positive samples with CA strip, the human rk39 strip and canine rk39 strip, the sensitivity was found of 90.0% (199/221), 65.2% (144/221) and 64.3% (142/221), respectively, all of which significantly differed from that of PCR method (χ2 = 141.76, 20.31, and 17.96, P < 0.05). The specificity of CA test strip and the two rk39 test strips in testing the blood samples of 316 dogs without Leishmania infection from the endemic areas was 95.3% (301/316), 99.7% (315/316) and 99.4% (314/316), respectively, all of which significantly differed from that of the standard (χ2 = 258.85, 312.01, and 308.05, P < 0.05). The positive-result coincidence rate of the CA strip and the canine and human rk39 strips with PCR in dogs with symptoms from the endemic areas was 88.5% (23/26), 88.5% (23/26) and 84.6% (22/26), respectively, all of which significantly differed from that of the PCR method (χ2 = 23.22, 23.22, and 20.50, P < 0.05). The positive-result coincidence rate of CA test strip and the canine and human rk39 test strips with PCR in dogs without symptoms in the endemic areas was 91.8% (179/195), 62.1% (121/195) and 61.5% (120/195), respectively, all of which significantly differed from that of the PCR method (χ2 = 136.25, 11.33, and 10.39, P < 0.05). Area under the curve (AUC) analysis was used to assess the method accuracy, showing the AUC value of all the 3 methods was above 0.8. The kappa consistency coefficient was 0.671-0.857, indicating that all of the three test strips are applicable for preliminary screening of Leishmania-infected dogs. The CA test strip is superior to the two rk39 test strips and could be a good method for screening Leishmania-infected dogs in endemic areas.

Key words： Leishmania; Immunochromatographic strip; Dog; Performance evaluation; Gansu Province

Cite this article

Da-wei YU , Fan LI , Yu FENG , Cheng-ming YANG , Jun-ke YANG , Lin-lin LIU , Yong-fu ZHANG . Evaluation of the performance of three immunochromatographic strips in detecting Leishmania infection in dogs in Gansu Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(6) : 792 -795 . DOI: 10.12140/j.issn.1000-7423.2020.06.021

References

[1] Boelaert M, Criel B, Leeuwenburg J, et al. Visceral leishmaniasis control: a public health perspective[J]. Trans Royal Soc Trop Med Hyg, 2000,94(5):465-471.
[2] Guan LR, Wang JY, Yang YT. Current challenges to leishmaniasis control in China[J]. Endem Dis Bull, 2003,18(1):46-49. (in Chinese)
[2] ( 管立人, 汪俊云, 杨玥涛. 中国利什曼病防治上的几个问题[J]. 地方病通报, 2003,18(1):46-49.)
[3] Gao CH, Wang JY, Yang YT, et al. Study on PCR method for detecting the asymptomatic infection of Leishmania infantum[J]. Chin J Parasitol Parasit Dis, 2006,24(2):92-96. (in Chinese)
[3] ( 高春花, 汪俊云, 杨玥涛, 等. PCR检测婴儿利什曼原虫无症状感染的研究[J]. 中国寄生虫学与寄生虫病杂志, 2006,24(2):92-96.)
[4] Laurenti MD, Rossi CN, Matta VL, et al. Asymptomatic dogs are highly competent to transmit Leishmania infantum chagasi to the natural vector[J]. Vet Parasitol, 2013,196(3):296-300.
[5] Bai XF, Guan YY, Wu WP. Advances in research using PCR to diagnose asymptomatic leishmaniasis[J]. J Pathog Biol, 2016,11(2):189-192. (in Chinese)
[5] ( 白雪飞, 官亚宜, 伍卫平. PCR技术在利什曼原虫无症状感染诊断中的应用研究进展[J]. 中国病原生物学杂志, 2016,11(2):189-192.)
[6] Wang JY, Feng Y, Gao CH, et al. Asymptomatic leishmania infection in human population of Wenxian County, Gansu Province[J]. Chin J Parasitol Parasit Dis, 2007,25(1):62-64. (in Chinese)
[6] ( 汪俊云, 冯宇, 高春花, 等. 甘肃省文县流行区人群婴儿利什曼原虫无症状感染现状[J]. 中国寄生虫学与寄生虫病杂志, 2007,25(1):62-64.)
[7] Bai XF, Guan YY, Wu WP. Advances in research using PCR to diagnose asymptomatic leishmaniasis[J]. J Pathog Biol, 2016,11(2):189-192. (in Chinese)
[7] ( 白雪飞, 官亚宜, 伍卫平. PCR技术在利什曼原虫无症状感染诊断中的应用研究进展[J]. 中国病原生物学杂志, 2016,11(2):189-192.)
[8] Mandal J, Dubey ML, Malla N, et al. Evaluation of direct agglutination test, rk39 test, and ELISA for the diagnosis of visceral leishmaniasis[J]. Am J Trop Med Hyg, 2008,79(1):76-78.
[9] Alves WA, Bevilacqua PD. Quality of diagnosis of canine visceral leishmaniasis in epidemiological surveys: an epidemic in Belo Horizonte, Minas Gerais, Brazil, 1993-1997[J]. Cad Saude Publica, 2004,20(1):259-265.
[10] Feng XP, Shi F, Yang YT, et al. Evaluation of colloid gold labeled immunochromatographic strip test for rapid detection of canine leishmanial infection in China[J]. Int J Med Parasit Dis, 2015,42(4):189-192. (in Chinese)
[10] ( 冯晓平, 石锋, 杨玥涛, 等. 一种胶体金免疫层析试条检测犬利什曼原虫感染的效果评价[J]. 国际医学寄生虫病杂志, 2015,42(4):189-192.)
[11] Saghrouni F, Ga?ed-Meksi S, Fathallah A, et al. Immunochromatographic rK39 strip test in the serodiagnosis of visceral leishmaniasis in Tunisia [J]. Trans Royal Soc Trop Med Hyg, 2009,103(12):1273-1278.
[12] Oliveira E, Saliba SW, de Andrade CF, et al. Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis[J]. Trans Royal Soc Trop Med Hyg, 2011,105(7):414-416.
[13] Yang YT, Shi F, Gao CH, et al. Establishment and evaluation of colloid gold labeled immunochromatographic strip test for rapid detection of visceral leishmaniasis[J]. Int J Med Parasit Dis, 2014,41(3):121-124. (in Chinese)
[13] ( 杨玥涛, 石锋, 高春花, 等. 快速检测内脏利什曼病特异抗体胶体金免疫层析试条方法的建立与效果评价[J]. 国际医学寄生虫病杂志, 2014,41(3):121-124.)
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