Clinical data of fascioliasis patients recorded in 6 prefectures(cities) in Yunnan Province were collected from November 2011 to November 2018. A total of 65 cases were enrolled, including 28 males and 37 females. The majority of them were farmers (64.6%, 42/65), being Han ethnicity (89.2%, 58/65), and coming from Dali District(72.3%, 47/65). All of the 65 cases had eaten raw vegetables, and 6 of them had drunk unboiled gully water. Eighteen cases involving 5 families had family aggregative phenomenon. All the cases visited hospital for suffering fever and abdominal pain. Blood routine tests showed increased eosinophilia; abdominal color ultrasound scanning or CT (and/or) MRI examination suggested varying degrees of organ lesion particularly in the liver; and fecal sample examination revealed a positive rate of Fasciola eggs of 23.1% (15/65). The test for serum antibody against Fasciola found 58 strongly positive and 7 positive, resulting in a positive rate of 100% (65/65). All of the cases showed obvious improvement in symptoms of fever and abdominal pain after treatment with trichlorobendazole, and blood routine tests showed that eosinophils and liver lesions gradually returned to normal.