CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2020 , Vol. 38 >Issue 3: 323 - 331

DOI: https://doi.org/10.12140/j.issn.1000-7423.2020.03.011

ORIGINAL ARTICLES

Analysis of genetic polymorphisms of merozoite surface protein-1 and circumsporozoite protein of Plasmodium vivax

  • Hang-yi JIN ,
  • Ling-ling ZHANG ,
  • Su-juan ZHU ,
  • Wei-min XU ,
  • Jun-fang CHEN ,
  • Wei RUAN ,
  • Li-nong YAO ,
  • Hua-liang CHEN
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  • 1 Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, China
    2 Nanjing Gulou County Center for Disease Control and Prevention, Nanjing 210003, China
    3 Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China

Received date: 2019-11-20

  Online published: 2020-07-07

Supported by

Hangzhou Agricultural and Social Development Research Initiative Design Project(20190101A12);Hangzhou Health and Family Planning Science and Technology Project(2018A79)

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Abstract

Objective This study aimed to conduct descriptive analysis of the genetic polymorphisms of Plasmodium vivax gene merozoite surface protein-1 (PvMSP-1) and circumsporozoite protein (PvCSP) in vivax malaria cases in Hangzhou City. Methods Blood samples and epidemiological information of vivax malaria cases reported in Hangzhou during 2008-2019 were collected. P. vivax genomic DNA was extracted by QIAamp DNA blood mini kit. Pvmsp-1 and Pvcsp were amplified by nested PCR and the amplification products were sequenced. The amino-acid sequences were analyzed using DNAStar and MEGA 6.0 softwares and the phylogenetic tree was constructed using the neighbor-joining method. The descriptive and inductive analysis of genetic polymorphism and place of infection origin were performed using SPSS 17.0. Results Fifty-six vivax malaria cases were sampled, of whom 44 and 54 samples were successfully sequenced for Pvmsp-1 and Pvcsp, respectively. Sequence analysis indicated that 44 Pvmsp-1 sequences were clustered into four genotypes: SalⅠ(18 samples), Belem (8), R-Ⅲ (17) and R-Ⅳ (1); 54 Pvcsp sequences were devided into two genotypes: VK210 (52 samples) and VK247 (2). Analysis of the genotyping and place of infection origin of Pvmsp-1 gene showed that the SalⅠtype was widely distributed in East China, Southeast Asia, India, and Ethiopia. The main type of Pvmsp-1 in East China was R-Ⅲ. In addition, the R-Ⅲ type was also distributed in South Asia and Southeast Asia. The Belem type was mainly distributed in Southeast Asia, South Asia and Ethiopia. Analysis of Pvcsp gene polymorphism and place of infection origin revealed four types of polymorphism with some regional features. Conclusion The Pvmsp-1 and Pvcsp genes in vivax malaria cases reported in Hangzhou during 2008-2019 were characterized being highly polymorphic, with some genotypes having certain regional features.

Key words： Plasmodium vivax; Merozoite surface protein-1; Circumsporozoite protein; Genetic diversity

Cite this article

Hang-yi JIN , Ling-ling ZHANG , Su-juan ZHU , Wei-min XU , Jun-fang CHEN , Wei RUAN , Li-nong YAO , Hua-liang CHEN . Analysis of genetic polymorphisms of merozoite surface protein-1 and circumsporozoite protein of Plasmodium vivax[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(3) : 323 -331 . DOI: 10.12140/j.issn.1000-7423.2020.03.011

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