To investigate the status of Toxoplasma infection in pregnant and postpartum women in Duyun district of Guizhou Province, China, serum samples were collected during 2015-2018 from pregnant and postpartum women receiving regular checkups and the women who had pathological pregnancies such as miscarriage, stillbirth, and deformity in Qiannan People′s Hospital, to test for specific IgM and IgG antibodies against Toxoplasma by ELISA. SPSS 17.0 software was used for statistical analysis. The positive rates of serum Toxoplasma antibody were compared by chi-square test. A total of 6 931 pregnant and postpartum women participated in the survey, including 6 825 normal pregnancies and parturients, and 106 pathological pregnancies and parturients. The age of the participants ranged 18-45 years, with ethnicities mainly of Han, Buyi, and Miao. The overall positive rate of Toxoplasma antibody was 6.1%(424/6 931), composed of 5.9%(403/6 825) in normal pregnant and postpartum women, and 19.8% (21/106) in pathological pregnancies and parturients (P < 0.01). There was a significant difference in the positive rate of Toxoplasma antibody among Han (7.5%, 215/2 886), Miao (5.4%, 132/2 453), Buyi (5.5%, 71/1 300), Shui (2.3%, 3/131), Tujia (2.0%, 2/100) and Dong (1.6%, 1/61) ethnicities (P < 0.05). There was also a significant difference in the positive rate of Toxoplasma antibody among cadres (1.3%, 22/1 714), teachers (1.8%, 22/1 216), rural workers (13.2%, 320/2 418) and other occupations (3.8%, 60/1 583) (P < 0.01). It was noted that the pregnant women with and without history of contacting with animals was 26.2% (361/1 379) and 1.1% (63/552), respectively, with the difference being statistically significant(P < 0.01). The results demonstrate the pregnant women with positive Toxoplasma antibody in Duyun District of Guizhou Province are mainly those having pathological pregnancyies, being Han ethnicity, engaging in rural farming work, and having contacts with animals.