Improvement in the Preparation of Paragonimus westermani Chromosome for Karyotype Analysis

›› 2002, Vol. 20 ›› Issue (3): 8-157.

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Improvement in the Preparation of Paragonimus westermani Chromosome for Karyotype Analysis

CHEN Shao hong;CHANG Zheng shan;CHEN Ming gang;ZHANG Yong nian;FENG Zheng

Institute of Parasitic Diseases;Chinese Center for Disease Control and Prevention *;Shanghai 200025

Received:1900-01-01 Revised:1900-01-01 Online:2002-06-30 Published:2002-06-30

Abstract

Abstract: 　Objective To improve Paragonimus westermani chromosome preparation technique. Methods After being exposed to colchicine, the gonadal cells of P.westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining. Results The chromosome number of P. westermani is 22,and the karyotype is 2m+6Sm+14t chromosome. Conclusion The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.

Key words: Paragonimus westermani, chromosome, karyotype

CHENShaohong;CHANGZhengshan;CHENMinggang;ZHANGYongnian;FENGZheng. Improvement in the Preparation of Paragonimus westermani Chromosome for Karyotype Analysis[J]. , 2002, 20(3): 8-157.

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Improvement in the Preparation of Paragonimus westermani Chromosome for Karyotype Analysis

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