›› 2003, Vol. 21 ›› Issue (6): 7-344.

• 论著 • Previous Articles     Next Articles

Application of Dot-immunogold Filtration Assay in Detection of IgG in Sera of Trichinellosis Patients

LIU Ying-jie~1,LIU Yun~1,QU Yao-hua~1,ZHENG Yong~2,LIU Yan-hong~3,ZHANG Yong-li~4,CHEN Juan~3
  

  1. 1 Cellular and Molecular Immunology Laboratory of Henan University,Kaifeng 475001 2 The First Affiliated Hospital of Henan University,Kaifeng 475001 3 Kaifeng Epidemic Prevention Station,Kaifeng 475000 4 Huaihe Affiliated Hospital of Henan University,Kaifeng 475000
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-12-30 Published:2003-12-30

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Abstract: 　Objective To study a new quick method for detecting serum IgG against Trichinella spiralis (T.s). Methods Membrane antigen of muscle larva of T.s was isolated and combined with the nitrocellulose membrane. Goat anti-human IgG was conjugated with the golden pellets of colloidal state which was used as marked antibody for the experiment of dot-immunogold filtration assay to examine specific IgG. Results T.s IgG and its corresponding antigen reacted on the membrane by filtration, and the result could be observed with naked eyes within 10 min. In the examination of 76 clinical serum samples, the positive rate was (94.7%). It was not significantly higher than that of ELISA(χ~2=2.83,P>0.05). Conclusion The dot-immunogold filtration assay is rapid and simple for performing and reading in the detection of IgG against T.s with high sensitivity and specificity.

Key words: trichinellosis, dot-immunogold filtration, ELISA, membrane antigen, immunodiagnosis