Infection and species identification of Anisakis larvae in sea fishes along Fujian coast

doi:10.12140/j.issn.1000-7423.2019.04.008

Abstract

Abstract:

Objective To understand the infection status and species of the larvae of Anisakis nematodes in sea fishes along Fujian coast. Methods The samples of marine fishes were randomly collected from three major fisheries along Fujian coast (Eastern, Middle and Southern) during January 2016 to December 2018. The fish species were identified and the Anisakis larvae were isolated from the visceral and muscle tissues of the infected fishes. The species of infected Anisakis spp. was examined and identified under dissecting microscope. The data of fish species, Anisakis species and infection rate were collected and analyzed. Results A total of 810 marine fishes classified as 32 species were examined, and the overall infection rate of Anisakis nematodes was 34.2% with average 11.2 worms in each infected fish. The fish species with highest infection rates of the nematode were Trichiurus lepturus (85.6%, 95/111), Scomberomorus niphonius (63.5%, 47/74), Ditrema temmincki Bleeker (36.7%, 67/162) and Plectorhynchus nitens (36.7%, 18/49), with average 23.1, 7.6, 4.5 and 4.2 larvae for each infected fish, respectively. The distribution of Anisakis larvae differed in different fish species, commonly seen in the mesenteric and intestinal wall, body cavity and gastric wall of those infected fishes followed by livers, pancreas and genitals, rarely seen in the muscle. Total four species of Anisakis nematodes were identified in the larvae collected from the fishes: Anisakis type Ⅰ, Anisakis type Ⅱ, Hysterothylacium amoyense and other Hysterothylacium. Conclusion The infection rate of Anisakis larvae in sea fishes along Fujian coast is high, and more prevention and control measures should be adopted and strengthened.

Key words: Anisakis spp., Infection rate, Species identification, Fujian Province

CLC Number:

R383.1

Chen-xin LIN, Shu-ling HUANG, Shi-han LIN, Dian-wei JIANG, Han-guo XIE. Infection and species identification of Anisakis larvae in sea fishes along Fujian coast[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(4): 417-421.

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References 17

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鱼种 Fish species	检查数/尾 No. exam’d	感染数/尾 No. infected	检获虫数 No. Anisakis	感染率/% Infection rate/%	感染度/条·鱼^-1 No. Anisakis per fish
带鱼Trichiurus lepturus	111	95	2 196	85.6	23.1
马鲛鱼Scomberomorus niphonius	74	47	358	63.5	7.6
海鲫鱼Ditrema temmincki Bleeker	162	67	303	41.7	4.5
包公鱼Plectorhynchus cinctus	49	18	75	36.7	4.2
斑鱾Goniistius zonatus	23	6	16	26.1	2.7
鱿鱼Squid	42	8	46	19.0	5.8
花尾胡椒鲷Plectorhinchus cinctus	12	2	5	16.7	2.5
春只Pseudosciaena crocea	37	6	28	16.2	4.7
黄姑鱼Nibea albiflora	34	5	13	14.7	2.6
鳙鱼Aristichthys nobilis	15	2	4	13.3	2.0
黄翅鱼Acanthopagrus latus	23	3	7	13.0	2.3
白鳞鱼 Ilisha elongata	17	2	2	11.8	1.0
鲈鱼Lateolabrax japonicue	31	2	7	6.5	3.5
黄瓜鱼Hypomesus olidus	57	2	3	3.5	1.5
鮸鱼Miichthys miiuy	20	7	18	35.0	2.6
小黄鱼Larimichthys polyactis	20	2	15	10.0	7.5
黄枝鱼Setipinna taty (Valenciennes)	3	1	2	33.3	2.0
龙头鱼Harpadon nehereus	5	1	3	20.0	3.0
白海鲫鱼 Carassius auratus cuvieri Temminck et Schlegel	1	1	2	100	2.0
三文鱼Oncorhynchus	20	0	0	0	0
鲭鱼Pneumatophorus japonicus	10	0	0	0	0
松江鲈鱼Trachidermus fasciatus	6	0	0	0	0
七纹豆娘鱼Abudefduf septemfasciatus	6	0	0	0	0
鲳鱼Stromateoides argenteus	6	0	0	0	0
金鲳鱼Trachinotus ovatus	5	0	0	0	0
章鱼Octopus	5	0	0	0	0
多宝鱼Panalichthys lethostigma	5	0	0	0	0
篮子鱼Siganus virgatus	4	0	0	0	0
金枪鱼Thunnus thynnus	3	0	0	0	0
虹鳟鱼Oncorhynchus mykiss	2	0	0	0	0
银鱼Hemisalanx prognathus	1	0	0	0	0
黑鲳鱼Parastromateus niger	1	0	0	0	0
合计Total	810	277	3 103	34.2	11.2

寄生部位Prasitic site	检获虫数/条（构成比/%） No. Anisakis larvae( Compositon ratio/%)
寄生部位Prasitic site	带鱼 T. lepturus	马鲛鱼 S. niphonius	海鲫鱼 C. auratus	包公鱼 H. nitens	合计 Total
体腔 Body cavity	867（39.5）	218（60.9）	189（62.4）	51（68.0）	1 325
肠系膜及肠壁Mesentery and intestinal wall	685（31.2）	87（24.3）	88（29.0）	18（24.0）	878
胃壁 Gastric wall	94（4.3）	29（8.1）	16（5.3）	3（4.0）	142
肝、胰 Liver and pancreas	31（1.4）	5（1.4）	4（1.3）	1（1.3）	41
生殖器 Genitals	516（23.5）	12（3.3）	6（2.0）	2（2.7）	536
肌肉 Muscle	3（0.1）	7（2.0）	0（0）	0（0）	10
合计Total	2 196（100）	358（100）	303（100）	75（100）	2 932