CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2017, Vol. 35 ›› Issue (1): 93-98.

• REVIEWS • Previous Articles    

Research progress on application of DNA barcoding technique in Culicidae taxonomy

Yu-yan GUO1, Lei LUO2, Xue-li ZHENG1,*()   

  1. 1 Department of Etiobiology, School of Public Health, South Medical University, Guangzhou 510515, China
    2 Department of Disinfection and Pesticide, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China
  • Received:2016-05-19 Online:2017-02-28 Published:2017-03-06
  • Contact: Xue-li ZHENG E-mail:zhengxueli2001@126.com
  • Supported by:
    Supported by the Scientific and Technological Projects of Guangdong Province(No. 2013B021800042);Scientific and Technological Projects of Guangzhou City(No. 2014Y2-00080)and Provincial Natural Science Fund of Guangdong(No. 2015A030313784)

Abstract:

DNA barcoding technique is a fast and accurate method for species identification. The DNA barcoding based on cytochrome c oxidase subunit Ⅰ (COⅠ) has recently been successfully applied for species identification of Culicidae. In this paper, we introduce the technique and principle of DNA barcoding, application and limitation of the technique based on CO I gene for species identification, as well as research development on applications of other molecular makers such as COⅡ, 16S RNA, and the first and the second internal transcribed spacers (ITS1 and ITS2) in species identification and to assist the COⅠ gene in identifying Culicidae species.

Key words: DNA barcode, Taxonomy, Culicidae, Molecular marker

CLC Number: