Abstract: A strain of axenic Naegleria fowleri was proliferated massively in Chang's medium and was introduced 'intranasally into 3 mice which were closely observed for 2-3 weeks. On the 13th day mouse 1 developed primary amoebic meningoencephalitis (PAME) symptoms, but mouse 2 had no signs of PAME at all at that time. On the 19th day, mouse 3 developed the same symptoms as mouse 1. Mice 1,2 and 4 were killed and autopsied for pathological examination. Mouse 3 was fixed in toto to keep the spasmodic posture.According to the typical manifestation, pathological findings and etiological characteristics, these mice were proved to be suitable models of Naegleria fowleri PAME.