Abstract: Essential -media tested were Earle, Earle +0.5% lactalbumin hydrolysate (Oxoid) and RPMI-1640. Baby jird testis and kidney tissue cell lines were used for culture. The most: favourable condition for the maintenance of the larvae in vitro was in a range of pH 6.4-7.5 and at temperatures of 36.5-37.5℃. The baby jird testis cell in growth medium RPMI-1640 was the best cell line tested with increases of 32.6% of the length and 36.8% of the width of the larvae taking place in 38 days cultivation. We observed successful moulting in both MT and MK cell lines. Brugia malayi began to moult on the tenth day. The survival of the larvae maintained on MK cell and MT cell were prolonged to 23 and 38 days respectively.