Abstract: A modification of the procedure described by Tsunematsu (1960) and Petrov (1974) for purification of Toxoplasma parasites ,was reported. Both pig and human strains of T. gondii maintained in mice by peritoneal inoculation were used. For parasite purification, peritoneal fluids were collected, digested with trypsin and filtered through a glass filter (G3) to remove host cells. Suspended parasites were freeze-dried for longterm preservation. Parasites and host cells were counted before and after digestion in some batches of infected mice, and Evan's blue staining was adopted for the examination of parasite vitality. Parasite yield, purity, viability as well as recovery rate were determined and compared in different lots.Application of this modified procedure resulted in an average parasite yield of (7.9±1.3) ×106 and (7.5±1.7) × 106 per mouse in pig and human strains respectively with no statistical difference (t = 0.55; p0.05). A high purity of 0.993±0.004 after treatment versus 0.668±0.18 before treatment was obtained, the noted difference being highly significant(t= 8.125; p0.01). The mean recovery rate as calculated from 4 lots has been 87.5% (t = 1.33; p0.05).