Abstract: Twenty-eight hybridoma cell lines secreting monoclonal antibodies (McAbs) against erythrocytic stage of P. falciparum were established by fusion of SP2/0 mice myeloma cells with spleen cells of BALB/c mice immunized with FCC-1/HN isolate of P. falciparum. Among these McAbs, 4 directed against schizonts only, 9 against merozoites and segments, 15 against late trophozoites and schizontes. By IFAT, 2 McAbs, 93D4 and 94B5, reacted only with homologous parasite, indicating that they might be strain-specific; while the other 26 McAbs reacted with both Hainan and Anhui isolates of P. jalciparum. Five McAbs (92A3, 94A2, 94A6, 94B3 and 96B5) cross-reacted with P. vivax. In addition, 96B5 was also cross-reactive with P. knowlesi and P. cynomolgi.