Abstract: Some biological and biochemical characteristics of metacercaria of Euparagonimus ce-nocopiosus obtained from crabs in the mountainous area of south Anhui, were compared with those of Paragonimus westermani from the same batch of crabs.The metacercariae of E. cenocopiosus prefer the location in heart rather than in the muscle of crabs. The average infection rate of crabs was 42.8% by E. cenocopiosus, while that by P. westermani was 100%. The infection rate of rats with E. cenocopiosus was 3/6 and 10/10 with P. westermani and the recovery rate of worm of these two species was 4.5-8.8% and 22-72.2% respectively. The number and pattern of protein bands of metacercaria of these two species by disc-PAGE and IEF technics were different.