Abstract: This paper reports an inhibitory ELISA using monoclonal antibodies M26-32 to detect malaria parasite antigen. The characteristic of this method is to coat the polysterene microculture wells with rabbit anti-mouse IgG serum instead of P. yoelii or P. falci-parum. Examination of 69 microscopically positive and 88 negative blood samples collec-ted on filter paper revealed that, taking inhibition late≥9% as positive standard, the coincidence rate for both microscopically positive and negative samples is above 90%. The inhibition rate is closely related to parasite density, the lowest detectable level being 2.6 parasites/104 erythrocytes.