Application of EITB in Immunodiagnosis of Cysticercosis

Abstract

Abstract:
　Objective To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis. Methods Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared. Results Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14 000 and 16 600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB. Conclusion A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.

Key words: cysticercosis, helminth antigens, immunoblotting, immunoelectrophoresis, two-dimensional, immunologic tests, ELISA

]WANGLi-na;GELing-yun;MIAOFeng;YUZhen-hua;LIUYu-bing;WANGLi-na;GELing-yun;MIAOFeng;YUZhen-hua;LIUYu-bing;ZHENTian-min;LIGui-ping;YANGShu-fang. Application of EITB in Immunodiagnosis of Cysticercosis[J]. , 2004, 22(2): 9-100.

[1]	WANG Wenya, LIU Jianfeng, ZHANG Mizhen, QIAN Peijun, LIU Liu, ZHAO Luyuan, LI Kerong, ZHANG Dongqi, LIU Yuhua, LIU Hongkun, CHEN Shaorong, ZHOU Changhai, QIAN Menbao, LI Shizhu. Analysis on Taenia infection and serum cysticercus antibody in population and their risk factors in Dali, Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(5): 615-622.
[2]	LI Na, ZANG Dare, WURI Lige, ALATENG Burigude, HAI Ying, HASI Surong. Fasciola spp. infection and molecular identification in cattle and sheep in Wushen Banner, Inner Mongolia [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 36-41.
[3]	LIU Wenhu, HUANG Ming, LIANG Jin, LIU Jianxiong, WEN Zhaomeng, MA Shaobo. A case of ventricular cysticercosis complicated with hydrocephalus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(5): 644-646.
[4]	CHEN Guoliang, WANG Liqun, LI Yanping, LIU Tingli, LI Hong, ZHANG Shaohua, LUO Xuenong, QIANG Wenjun. Establishment and application of a rolling circle amplification method based on novel-miR1 for detection of Cysticercus pisiformis infection in rabbit [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 294-299.
[5]	FANG Wen, YANG Jing, WANG Haiying, CHEN Shaorong, LIU Yuhua, LI Tianmei. Excystment conditions and excysted metacercaria morphological observation of Fasciola hepatica [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 198-201.
[6]	DUAN Yueli, YU Yiqi, ZHU Haoxiang, WANG Xinyu. A case of atypical cerebral cysticercosis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 128-130.
[7]	CAO Tian-xing, LIU Jun-long, ZHANG Zhi-gang, SHI Kang-yan, SHI Miao, GUAN Gui-quan, LI You-quan, YIN Hong, LUO Jian-xun. Prokaryotic expression of Theileria annulata recombinant TA04380 protein and establishment of ELISA [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 362-368.
[8]	ZHOU Yan, CHEN Shao-hong, LI Hao, CHENG Na, HONG Jia-lin, XU Xue-nian. Cloning, expression of the thioredoxin peroxidase gene of Paragonimus westermani and its immunodiagnostic potential [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(1): 20-26.
[9]	DENG Yan1, ZHANG Ya-lan1, LI Su-hua1, CHEN Wei-qi1, LIN Xi-meng1, CHEN Xi2, WANG Ting-zhu3, LI Peng1, ZHANG Hong-wei1, XU bian-li1*. A KAP survey on taeniasis and cysticercosis in Fangcheng County of Henan Province in 2016 [J]. , 2018, 36(3): 19-242-245.
[10]	Jin LI, Yan WANG, Qing-kuan WEI, Feng-ju JIA, Ting XIAO, Hui SUN, Zhen-hua YU, Bing-cheng HUANG. Screening of antigens specific for the anti-Cysticercus cellulosae IgG4 antibody [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(5): 509-511.
[11]	Le-sheng ZHANG, Lei SUN, Yuan HU, Wen-ci GONG, Yan-juan WANG, yu-juan SHEN, Yu-xin XU, Tian-ping WANG, Jian-ping CAO. Preparation and identification of monoclonal antibodies targeting Giardia lamblia [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(2): 160-163.
[12]	YU Qin1, ZHU Yan-hong2, GUAN Fei3, YANG Hai2. Establishment of Magnetic Affinity Enzyme Linked Immunoassay Based on Schistosoma japonicum* Recombinant Antigen Sj26 and Its Application in Detection of Serum Antibody with Low Intensity of Infection [J]. , 2016, 34(4): 2-303-307.
[13]	LIU Ying, HU Tian-yu, ZHONG Ce-jun, LV Xiao-ju*. Clinical Analysis of 94 Patients with Neurocysticercosis in Sichuan Province [J]. , 2016, 34(2): 6-118-122.
[14]	YANG Yi, YANG Yue-tao, SHI Feng, GAO Chun-hua, WANG Jun-yun*. Analysis of Antibodies with Superior Antigen-recognition in Serum from Patients Infected by Necator americanus [J]. , 2016, 34(1): 7-40-45.
[15]	LIU Wen-yuan1, 2，ZHOU Jiang-jin3. Allergen Detection among 2 769 Children with Allergic Symptoms in Ningbo Area [J]. , 2013, 31(4): 19-327-328.

Application of EITB in Immunodiagnosis of Cysticercosis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments