Abstract: In this paper, the results of dot-immunobinding assay (DIA) in tne diagnosis of falciparum malaria were reported. Among 53 sera from confirmed falciparum malaria cases from Hainan, 96,2%(51/53) showed positive reactions, while none of the 42 serum samples from blood donors in Shanghai and Ningxia Hui Autonomous Region where malaria was absent was found positive (Table 1). No cross reaction was observed with sera from cases of schistosomiasis, visceral leishmaniasis, paragonimiasis and fasciolopsiasis buski. The positive rate of DIA is higher than that of conventional ELISA(84.9%) or IFA(83.0%). It is recommended as a serological test of choice for the diagnosis of fal-ciparum malaria.