Abstract: This article presents the changes in the concentration of bilirubin, cholesterol, phos-pholipid, bile acid and the activity of bacterial β-glucuronidase in hepatic bile of rabbits infected with metacercariae of Clonorchis sinensis. We found that the activity of bacterial β-glucuronidase was significantly higher in the infected group (52.6 ±31.6u/dl) than that in control rabbits (12.5±20.5 u/dl) (P0.001), and no marked changes in the concentration of bilirubin, cholesterol, phospholipid and bile acid were found. By using alcian blue-PAS combined stain method the authors also noticed that the amount of gly-coprotein in gobletcells has considerably increased and secreted increasingly to the biliary duct after infection with Clonorchis sinensis. As the increase in bacterial β-glucuronidase activity and glycoprotein in bile is in favour of the formation of pigment stone, this may explain why clonorchiasis is often complicated with cholelithiasis.