Abstract: Schistosoma japonicum adult worm 31/32 kDa proteins (Sj 31/32) were separated on polyacrylamide slab gels and purified by electrophoretic elution. These purified proteins were used to immunize mice in order to observe their protective immunity against challenge. The results of SDS-PAGE,EITB and ELISA indicated that the 31/32 kDa proteins separated and purified by means of these methods were pure and active. It was assumed that Sj 31/32 proteins could reduce worm burden and inhibit the fecundity of schistosome and formation of egg granuloma. The results suggested that 31/32 kDa 5. japonicum proteins might be an important component of a multivalent vaccine against schistosomiasis japonica (Figs. 1 - 4).