Abstract:

The Taenia asiatica eggs pre-incubated with sodium hypochlorite solution for 4 min, 6 min and 8 mins were subcutaneously injected into mice with normal immune function（groups A1-A3 respectively, n=20 in each） and mice with immunosuppression（groups B1-B3, n=20 in each）. All groups of mice began to show body discomfort on day 5 after infection and develop lumps on the back about on day 15. In groups A1-A3, animal death occurred during days 7-15, with a same survival rate of 95.0%（19/20） and infection rate of 89.4%（17/19）, 73.6%（14/19） and 47.3%（9/19） respectively. In groups B1-B3, animal death occurred during days 7-50, with survival rate of 60%（13/20）, 55%（11/20）and 55%（11/20） and infection rate of 76.9%(10/13), 54.5%(6/11) and 45.4%(5/11) respectively. After the scolex of cysticercus was evaginated with 15% pig bile, four suckers, an apparent rostellum and two distinct hook-like puncta structures were seen. These results indicate that mice with normal immune function can be used as a replacement of immunosuppressive mice to establish a T. asiatica oncosphere infection model. In addition, the T. asiatica eggs pre-incubated with sodium hypochlorite solution for 4 min have the strongest infection ability.

Key words: Taenia asiatica, Mouse model, Normal immune function, Oncosphere, Hatch