Abstract: Objective  To evaluate biological safety of Aspergillus fumigatus SL-30, a potential molluscicide, to non-target organisms.  Methods  A. fumigatus SL-30 spores were scattered in the water (200 ml) from Yangtze River, lake, rain and tap water to forming 6×106 cfu/ml, the number of spores were then determined and recorded every 2 days. Effect of A. fumigatus SL-30 spores with concentration ranging from 104 cfu/ml to 106 cfu/ml on Brachydanio rerio, Macrobrachium nippoensis and tadpoles of Rana limnochris was tested by standard laboratory procedure, and survival rate of the above animals in 30 days was recorded. The tests included acute oral toxicity in mice, acute dermal toxicity and acute inhalation toxicity in rats.  Results  Spores of A. fumigatus SL-30 can survive for about 12 days in each water samples. Under the spore concentration of 104 cfu/ml, 105 cfu/ml and 106 cfu/ml, there was no significant dose-dependent relationship between spore concentration and survival rate of experiment animals. No significant difference in survival rate was found between the three kinds of aquatic organisms treated with A. fumigatus SL-30 and de-chlorinous water（P＞0.05）. According to the experiment results of acute oral toxicity, acute dermal toxicity and acute inhalation toxicity, the acute toxicity of A. fumigatus SL-30 on animal was low. And the animals in experiment group did not show any obvious poisoning symptoms, anatomical abnormalities and pathologic change of the tissues.  Conclusion  Aspergillus fumigatus SL-30 is comparatively safe to environment and tested non-target organisms.

Key words: Aspergillus fumigatus, Non-target organism, Biological security, Molluscicide