Combined Expression of TSO45W-4BX from Taenia solium and Porcine CD58 in Escherichia coli

›› 2006, Vol. 24 ›› Issue (4): 11-289.

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Combined Expression of TSO45W-4BX from Taenia solium and Porcine CD58 in Escherichia coli

LUO Xue-nong;ZHENG Ya-dong;DOU Yong-xi; HOU Jun-lin;JING Zhi-zhong; CAI Xue-peng

Key Laboratory of Veterinary Parasitology of Gansu Province， State Key Laboratory of Veterinary Etiological Biology， CAAS， Lanzhou 730046， China

Received:1900-01-01 Revised:1900-01-01 Online:2006-08-30 Published:2006-08-30
Contact: CAI Xue-peng

Abstract

Abstract: Objective To express the TSO45W-4BX of Taenia solium in combination with CD58 as a molecular adjuvant for improving the protective efficacy of the TSO45W-4BX recombinant vaccine. Methods TSO45W-4BX and porcine CD58 genes were amplified by PCR， using recombinant plasmids pGEM-4B and pGEM-CD58 as template respectively. The CD58 fragment was inserted into the recombinant plasmid pGEX-4T-1 with directly ligated TSO45W-4BX. The transformant was induced with IPTG and followed by identifying the integrity of the recombinant containing TSO45W-4BX and porcine CD58 with PCR and sequencing. The products were analyzed by SDS-PAGE and Western blotting. Results The expression products of Mr 69 000 GST-4BX/CD58 and Mr 41 000 GST-4BX were present mainly in the form of inclusion bodies and soluble substance respectively， and both were recognized by sera of cysticercosis patients. Conclusions The TSO45W-4BX co-expressed with porcine CD58 conserves its immune reactivity.

Key words: Taenia solium, TSO45W-4BX, CD58, Combined expression

LUOXue-nong;ZHENGYa-dong;DOUYong-xi;HOUJun-lin;JINGZhi-zhong;CAIXue-peng. Combined Expression of TSO45W-4BX from Taenia solium and Porcine CD58 in Escherichia coli[J]. , 2006, 24(4): 11-289.

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Combined Expression of TSO45W-4BX from Taenia solium and Porcine CD58 in Escherichia coli

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