Abstract: AIM:To evaluate the diagnostic value of four specific antigens from cDNA of
Cysticercus cellulosae . cC1,cC2,and cP1 and cH1 ( 28 kDa, 18 kDa, 14 kDa and 34 kDa ), mixing in equal proportions for the diagnosis of cysticercosis. METHODS:Taking the FP (fusion proteins) as antigen to make IWB (improved Western blot ) analysis basing on the detection of antibody
responses against FP,and making ELISA / IHA crude antigen(CA) analysis.They were evaluated
comparatively while using l07 infected sera of cysticerco sis cases, 40 infected sera of clonorchiasis cases, 24 infected sera of ech inococcosis cases and 34 sera of healthy persons. The FP are encoded by cDNAs of β-galacto sidase-specific antigens of Cysticercus cellulosae isolated from the cDNA library. RESULTS: 94 (87. 9% ) sera from l07 cysticercosis cases recognized FP in IWB and could not cross-react with the sera of echinococcosis cases, clonorchiasis cases and healthy persons, the specific rates were 100%, whereas ELISA, IHA using CA were 84. 1%and 74. 8%, respectively and could cross-react with the sera of echinococcosis cases, the false positive rates were 2. 5% and 12. 5% respectively; CA 2EL ISA/IHA could cross-react with the sera of clonorchiasis patients, the false positive rates were 8.3% and 16. 7%, respectively; and they could also cross-react with the sera of healthy persons, the false positive rates were 8. 8% and 11. 8% , respectively. CONCLUSION: The recombinant FP used in the immunodiagnosis of cysticercosis is specific and sensitive.

Key words: Cysticercosis, immunodiagnosis, fusion protein, improved Western blot