Analysis on laboratory diagnosis efficacy and reexamination of the results for imported malaria cases in Tai’an City of Shandong Province during 2015—2019

doi:10.12140/j.issn.1000-7423.2021.01.020

Abstract

Abstract:

The imported malaria cases in Tai’an City, Shandong Province, during 2015—2019 were diagnosed and the results rechecked at Province-, city-, and county-based laboratories using microscopy, nested-PCR, and colloidal gold methods, respectively. A total of 122 imported malaria cases were diagnosed and the results rechecked by the city laboratory. Rechecking the results, the provincial malaria diagnosis reference laboratory confirmed 122 cases positive, among them, the coincidence rate by microscopy, colloidal gold and PCR methods was 94.3% (115/122), 95.9% (117/122), and 100%, respectively; 7 cases of microscopy negative were falciparum malaria; while of 5 cases of colloidal gold method negative, 2 cases were falciparum, and 3 non-falciparum. There was a significant difference in the coincidence rate among the three examination methods (P < 0.05). In addition, there was a significant difference in the coincidence rate of laboratory diagnosis for four plasmodium species (P < 0.01), among them, the diagnosis coincidence rate for Plasmodium falciparum and P. vivax was highest (100%), whereas the lowest coincidence was seen in P. ovale (12/18). The pass rate of diagnosis at the first visit in medical institutions was 94.3% (115/122), and the pass rate of rechecking at city-level and county-level centers for disease control (CDC) were 97.5% (119/122) and 95.1% (116/122), respectively (P > 0.05). It is necessary to further strengthen skill training for laboratory microscopy examiners and actively carry out malaria nucleic acid testing, in order to improve the performance level of diagnosis in medical institutions and CDCs.

Key words: Malaria, Imported cases, Microscopy, Nested-PCR, Colloidal gold method, Accuracy

CLC Number:

R531.3

ZHANG Xin-feng, LIU Yun-qiang, ZHANG Zhi-jun, XU Yan, LI Jin, ZHANG Ben-guang. Analysis on laboratory diagnosis efficacy and reexamination of the results for imported malaria cases in Tai’an City of Shandong Province during 2015—2019[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(1): 129-132.

Figures/Tables 3

References 14

[1]	Grant EG, Benson CB, Moneta GL, et al. Carotid artery stenosis: grayscale and doppler ultrasound diagnosis: society of radiologists in ultrasound consensus conference[J]. Ultrasound Q, 2003,19(4):190-198. doi: 10.1097/00013644-200312000-00005 pmid: 14730262
[2]	Du WP, Zhang LL, Zhang XF, et al. Epidemiological analysis of imported malaria in Tai’an City of Shandong Province during 2011—2015[J]. Chin J Parasitol Parasit Dis, 2016,34(6):505-507. (in Chinese)
	( 杜卫萍, 张林林, 张新峰, 等. 2011—2015年山东省泰安市输入性疟疾疫情分析[J]. 中国寄生虫学与寄生虫病杂志, 2016,34(6):505-507.)
[3]	National Health and Family Planning Commission of the People's Republic of China. Diagnosis of malaria: WS 259—2015[S]. Beijing: Standards Press of China, 2016. ( in Chinese)
	( 中华人民共和国国家卫生和计划生育委员会. 疟疾的诊断: WS 259-2015[S]. 北京: 中国标准出版社, 2016.)
[4]	Huang BC, Xu C, Li J, et al. Application of nested PCR in diagnosis of imported ovale malaria[J]. Chin J Parasitol Parasitol, 2015,33(1):49-51, 57. (in Chinese)
	( 黄炳成, 徐超, 李瑾, 等. 巢式PCR技术在输入性卵形疟诊断中的应用研究[J]. 中国寄生虫学与寄生虫病杂志, 2015,33(1):49-51, 57.)
[5]	Huang GQ, Yuan FY, Hu LQ, et al. Prevalent situation of malaria in 2008 in Hubei Province[J]. J Public Health Prev Med, 2009,20(6):6-8. (in Chinese)
	( 黄光全, 袁方玉, 胡乐群, 等. 湖北省2008年疟疾流行态势分析[J]. 公共卫生与预防医学, 2009,20(6):6-8.)
[6]	Wang SQ, Zhou XN. Standardize malaria detection to help eliminate malaria in China: the compliation background and standard explanation of microscopic examination of blood films for malaria parasites[J]. China Health Standard Manag, 2017,8(28):4-7. (in Chinese)
	( 王善青, 周晓农. 规范疟原虫检测助力疟疾消除——《疟原虫检测血涂片镜检法》标准编制背景与解读[J]. 中国卫生标准管理, 2017,8(28):4-7.)
[7]	Wu DN, Sun LC, Zhang HX, et al. Evaluation of nested PCR in laboratory diagnosis of imported Plasmodium falciparum[J]. J Trop Med, 2019,19(1):13-15, 20. (in Chinese)
	( 吴冬妮, 孙凌聪, 张华勋, 等. 巢式PCR在输入性三日疟实验室诊断中的效果评价[J]. 热带医学杂志, 2019,19(1):13-15, 20.)
[8]	Xiao LL, Liu Y, Wang J, et al. Integrated detection of imported vivax malaria at Beijing Port[J]. Chin J Front Health Quar, 2019,42(5):328-331. (in Chinese)
	( 肖利力, 刘翌, 王瑾, 等. 北京口岸一例输入性间日疟的综合检测[J]. 中国国境卫生检疫杂志, 2019,42(5):328-331.)
[9]	She DY, Wu JH, Lu LD, et al. Evaluation of detection capability of malaria laboratories in Guizhou Province (prefecture level) from 2014 to 2016[J]. Chin J Parasitol Parasitol, 2017,35(5):466-471. (in Chinese)
	( 佘丹娅, 吴家红, 卢丽丹, 等. 2014—2016年贵州省市(州)级疟疾实验室检测能力的评价[J]. 中国寄生虫学与寄生虫病杂志, 2017,35(5):466-471.)
[10]	Wang ZY, Jiang L, Zhang YG, et al. Comparative analysis of malaria detection ability of laboratories in Shanghai City from 2012 to 2015[J]. Chin J Schisto Control, 2017,29(3):305-309, 328. (in Chinese)
	( 王真瑜, 江莉, 张耀光, 等. 2012—2015年上海市实验室疟疾检测能力比较分析[J]. 中国血吸虫病防治杂志, 2017,29(3):305-309, 328.)
[11]	Gu YP, Zhu GD, Zhang C, et al. Quality analysis of malaria microscopic examinations in Jiangsu Province from 2011 to 2017[J]. Chin J Schisto Control, 2018,30(4):369-373. (in Chinese)
	( 顾亚萍, 朱国鼎, 张超, 等. 2011—2017年江苏省疟疾镜检工作质量分析[J]. 中国血吸虫病防治杂志, 2018,30(4):369-373.)
[12]	Zhou XW, Zhou HM, Yang YM, et al. Results of microscopic examinations and blood slide re-examinations of malaria elimination evaluation in ten prefectures of Yunnan Province[J]. Chin J Sschisto Control, 2019,31(5):543-545, 551. (in Chinese)
	( 周兴武, 周红梅, 杨亚明, 等. 云南省10州(市)消除疟疾考核评估镜检和血片复核结果分析[J]. 中国血吸虫病防治杂志, 2019,31(5):543-545, 551.)
[13]	Collins WE, Jeffery GM. Plasmodium ovale: parasite and disease[J]. Clin Microbiol Rev, 2005,18(3):570-581.
[14]	Wang YB, Kong XL, Zhang BG, et al. Epidemiological analysis of malaria prevalence in Shandong Province in 2012[J]. Chin J Schisto Control, 2013,25(6):618-622. (in Chinese)
	( 王用斌, 孔祥礼, 张本光, 等. 2012年山东省疟疾疫情流行病学特征分析[J]. 中国血吸虫病防治杂志, 2013,25(6):618-622.)

年份	恶性疟			间日疟			卵形疟			三日疟			合计
年份	病例数	正确例数	符合率/%	病例数	正确例数	符合率/%	病例数	正确例数	符合率/%	病例数	正确例数	符合率/%	病例数	正确例数	符合率/%
2015	25	25	100	5	5	5/5	5	4	4/5	5	4	4/5	40	38	95.0
2016	19	19	19/19	2	2	2/2	5	3	3/5	0	0	/	26	24	92.3
2017	12	12	12/12	4	4	4/4	3	2	2/3	1	1	1/1	20	19	95.0
2018	14	14	14/14	0	0	/	4	2	2/4	0	0	/	18	16	16/18
2019	15	15	15/15	1	1	1/1	1	1	1/1	1	1	1/1	18	18	18/18
合计	85	85	100	12	12	12/12	18	12	12/18	7	6	6/7	122	115	94.3

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[2]	WEI Luanting, LI Runze, GUAN Liangchao, ZHANG Qianyu, LI Cheng, CAO Yaming, ZHAO Yan. Research progress of antimalarial drugs [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 486-491.
[3]	ZHANG Le, XIA Jiawei, LI Xiang, MA Zhongxu, JIANG Jianjie, TANG Yalin, LIU Shu, ZHANG Kaiyi. Clinical analysis of imported COVID-19 cases complicated with severe falciparum malaria [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(4): 506-509.
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[5]	CAO Wei, WANG Yi, ZHANG Xizhi, TONG Guodong, YANG Chao, SHEN Yan, ZHAO Ya. Research progress in adjunctive therapy of cerebral malaria [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 361-373.
[6]	GENG Yan, LAN Ziyao, LI Yang, DAI Jiarui, CAI Shan, LU Lidan, HUANG Yuting, SHI Weifang, SHE Danya. Epidemiological analysis of malaria in Guizhou Province from 2017 to 2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 384-388.
[7]	ZHANG Li, YI Boyu, YIN Jianhai, XIA Zhigui. Epidemiological characteristics of malaria in China, 2022 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 137-141.
[8]	CHEN Zhuyun, OUYANG Rong, XIAO Lizhen, LIN Yaoying, XIE Hanguo, ZHANG Shanying. Current status of the primary surveillance and response system during the post malaria elimination phase in Fujian Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 170-175.
[9]	SUN Jun. The biological significance of malarial hemozoin’s formation [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 209-212.
[10]	CHEN Zhihui, HONG Jing, ZHANG Rongbing, YANG Qian, YE Qing, LI Jianrong, TIAN Rong. Epidemiological analysis on malaria cases reported in Kunming during 2006—2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 233-237.
[11]	LIU Jiancheng, XU Yan, WANG Longjiang, KONG Xiangli, WANG Yongbin, LI Yuejin. Surveillance on imported malaria in Linyi City of Shandong Province from 2015 to 2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 249-252.
[12]	ZHANG Yaoguang, JIANG Li, WANG Zhenyu, ZHU Min, ZHU Qian, MA Xiaojiang, YU Qing, Chen Jian. Analysis of the causes of misdiagnosis of seven imported malaria cases in Shanghai from 2020 to 2021 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 68-74.
[13]	LI Su-hua, JI Peng-hui, ZHOU Rui-min, HE Zhi-quan, QIAN Dan, YANG Cheng-yun, LIU Ying, LU De-ling, WANG Hao, ZHANG Hong-wei, ZHAO Yu-ling. Appraisal of diagnosis capacity of malaria reference laboratories in Henan Province during 2015—2019 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(6): 748-753.
[14]	LI Mei, ZHOU He-jun, XIA Zhi-gui, ZHANG Li, TU Hong, YIN Jian-hai. Quality evaluation on the preparation of the malaria blood smears at the national level in 2019 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(6): 754-759.
[15]	ZHAO Hui, XIANG Zheng, ZHOU Long-can, PAN Mao-hua, YANG Zhao-qing. Research progress of amodiaquine as an antimalarial drug [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(6): 786-791.