Abstract: 　AIM: To construct an expression vector,including a chimeric cDNA of porcine IFN-γ and Cysticercus cellulosae antigen cC1. METHODS: DNA fragments of porcine IFN-γ and cC1 including linkers were generated by polymerase chain reaction(PCR).The recombinant vector pJLA-PRcC1 was constructed by inserting a chimeric cDNA of porcine IFN-γ and Cysticercus cellulosae antigen cC1,and transformed to E.coli XL-Blue. RESULTS: The recombinant vector was identified by restriction analysis.An inserted fragment about 1.5 kb could be found.After induction,a 52 kDa new protein band appeared in SDS-PAGE. CONCLUSION: The fusion expression of porcine IFN-γ and cC1 antigen in Escherichia coli cells is successful.\;

Key words: Cysticercosis, fusion protein, IFN-γ