SEQUENCING OF CYTOCHROME C OXIDASE 1 GENE OF ANCYLOSTOMA DUODENALE AND NECATOR AMERICANUS *

›› 1999, Vol. 17 ›› Issue (2): 5-83.

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SEQUENCING OF CYTOCHROME C OXIDASE 1 GENE OF ANCYLOSTOMA DUODENALE AND NECATOR AMERICANUS *

LI Tiehua¹;ZHAN Bin ¹;John M Hawdon ²;GONG Xin ¹;XIAO Shuhua ¹ SHAN Qiang ¹;FENG Zheng 1;Peter J Hotez ²

1 Institute of Parasitic Diseases;Chinese Academy of Preventive Medicine **;Shanghai 200025 2 Medical Helminthology Labora

Received:1900-01-01 Revised:1900-01-01 Online:1999-04-30 Published:1999-04-30

Abstract

Abstract: 　AIM: To identify the genetic diversity between Ancylostoma duodenale and Necator americanus. METHODS : Mitochondrial cytochrome C oxidase subunit 1 (CO1) gene was amplified from genomic DNA of human hookworms collected from infected patients in Hejiang County, Sichuan Province , and the purified PCR products were directly sequenced by using Licor auto sequencer. RESULTS: The PCR products were about 700 bp. Alignment of CO1 gene fragment sequences showed 89 7% similarity between Ancylostoma duodenale and Necator americanus, but still certain nucleotide variations (10 3%) existed. CONCLUSION: CO1 gene sequence can be used as a marker to identify the two species of human hookworms.

Key words: Cytochrome C oxidase1, DNA sequence, Necator americanus, Ancylostoma duodenale

LITiehua;ZHANBin;JohnMHawdon;GONGXin;XIAOShuhuaSHANQiang;FENGZheng;PeterJHotez . SEQUENCING OF CYTOCHROME C OXIDASE 1 GENE OF ANCYLOSTOMA DUODENALE AND NECATOR AMERICANUS *[J]. , 1999, 17(2): 5-83.

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SEQUENCING OF CYTOCHROME C OXIDASE 1 GENE OF ANCYLOSTOMA DUODENALE AND NECATOR AMERICANUS *

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