Abstract: In order to improve diagnostic method to Babesia gibsoni infection, gene recombination technique was used in our study. By this technique, we constructed and expressed recombinant apical membrane antigen-1 （BgAMA-1）, and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen. The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test. These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs.

Key words: Babesia gibsoni, BgAMA-1, ELISA