关键词: 弓形虫病, 寡核苷酸引物, 聚合酶链反应, DNA诊断

Abstract: Based on the partial sequences of the specific DNA cloned fragment from T.gondii (ZS2 strain) a specific primer of the oligonudeotide for the Toxoplasma gondii DNA sequence has been designed and synthesized in our laboratory.The method of the DNA diagnosis for to-xoplasmosis by polymerase chain reaction (PCR) has been established.A specific amplified band was shown in the PCR products from DNAs of T.gondii and seven manifold terata.The DNAs from the peripheral blood leukocytes of fifty normal individuals and seventy-five patients as infants with hepatitis syndrome and pregnant women with previous abnormal birth histories were diagnosed by PCR.Among the seventy-five diagnosed cases,ten were positive.The normal individuals all were negative.Using 32P-cloned T.gondii specific DNA fragment as probe and Southern blot assay,the results showed that the probe only hybridized to the speciific amplified DNA bands,but did not hybridize to the amplified DNA products of negative cases.Our PCR method is a rapid,highly specific and sensitive one for delecting toxoplasmosis as compared with DNA probing,immunoassay and animal inoculation.