中国寄生虫学与寄生虫病杂志 ›› 2010, Vol. 28 ›› Issue (4): 17-267.

• 研究简报 • 上一篇    下一篇

中缅边境间日疟原虫环子孢子蛋白基因分型

徐贵全1,张再兴2 *,李娜3,代宏剑4   

  1. 1 贵阳市第四人民医院,贵州 550002;2 云南省寄生虫病防治所,思茅 650034;3 四川大学华西公共卫生学院统计教研室,四川610041;4 昆明市儿童医院,昆明 650034
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-30 发布日期:2010-08-30

Genotyping of Plasmodium vivax in China-Myanmar Border by Circumsporozoite Protein

XU Gui-Quan1,ZHANG Zai-xing2 *,LI Na3,DAI Hong-jian4   

  1. 1 The Fourth People's Hospital of Guiyang,Guizhou 550002,China;2 Yunnan Institute of Parasitic Diseases,Simao 665000,China;3 HuaXi Public Health Institute,Sichuan University,Chengdu 610041,China;4 Kunming Children’s Hospital,Kunming 650034,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-30 Published:2010-08-30

摘要: 根据间日疟原虫环子孢子蛋白(CSP)基因设计特异分型引物,利用巢式PCR技术(Nested?鄄PCR)对采自中缅边境的间日疟患者血样作分型鉴定。检出的174份血样中,PV-I型热带族占54.6%(95/174)、温带族占35.6%(62/174)、PV-II型占2.9%(5/174)、混和感染占6.9%(12/174)。表明中缅边境的间日疟原虫存在4种基因型,以热带族为优势虫株,缅甸拉咱与云南腾冲间日疟原虫CSP基因型构成无差异(χ2=3.381, P>0.05)。

关键词: 间日疟原虫, 环子孢子蛋白, 基因分型

Abstract: By using a series of type-specific primers for Plasmodium vivax circumsporozoite protein (CSP) gene and nested PCR, genotyping was conducted for the specimens of Plasmodium vivax isolated from China-Myanmar border. In 174 isolates of P. vivax, four genotypes, namely, tropical zone family strain, temperate zone family strain, genotype-mixed infection and PV-II type, were identified each accounting for 54.6 %, 35.6%, 6.9%, and 2.9%, respectively. The tropical zone family strain was dominant in the border area. There was no significant difference on the P.v CSP genotype constitution between Laza isolate of Myanmar and Tengchong isolate of Yunnan, China

Key words: Plasmodium vivax, Circumsporozoite protein, Genotyping