中国利什曼原虫分离株分子核型分析

中国寄生虫学与寄生虫病杂志 ›› 1998, Vol. 16 ›› Issue (6): 425-431.

中国利什曼原虫分离株分子核型分析

吴波; 瞿靖琦

中国预防医学科学院寄生虫病研究所

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-12-31 发布日期:1998-12-31

KARYOTYPE ANALYSIS OF LEISHMANIA ISOLATES IN CHINA *

Wu Bo; Qu Jingqi

Institute of Parasitic Diseases; Chinese Academy of Preventive Medicine**; Shanghai 200025

Received:1900-01-01 Revised:1900-01-01 Online:1998-12-31 Published:1998-12-31

摘要/Abstract

摘要： 目的：通过核型分析探讨中国利什曼原虫分离株遗传变异情况。方法：应用脉冲电泳技术对16株中国利什曼原虫分离株基因组核型进行分析。结果：被测分离株分有14条－26条染色体（低于2200kb）被区分，核型差异大多集中于225kb－850kb染色体区域，较大染色体相对保守。其中14株属于杜氏利什曼原虫复合体的分离株存在明显的种内核型差异，但来自同一流行区的分离株核型显示差异较小。所有杜氏利什曼原虫复合体的分离株均含有11条相似染色体带：320kb,350kb,380kb,870kb,910kb,1070kb， 200 kb, 1 250 kb, 1 350 kb, 1 600 kb 和1 900 kb。其中, 从白蛉体内分离出的771 分离株核型显示较为独特, 共分离出26 条染色体带。另两株从大沙鼠体内分离的、先前已分被命名为沙鼠利什曼原虫和都兰利什曼原虫的R12 和R20 分离株显示相近核型。结论: 中国杜氏利什曼原虫复合体内存在明显种内遗传变异, 但亲缘关系越近的分离株核型也越相近。地理环境与媒介白蛉均对杜氏利什曼原虫复合体内遗传变异起一定作用。R12 和R 20 分离株倾向于属于同一个种或两个亲缘关系极近的种。同时, 可见寄生的哺乳动物宿主不同可能与利什曼原虫遗传关系密切。

关键词: 利什曼原虫, 脉冲电泳, 分子核型

Abstract: AIM: To further understand the genetic variation among Leishmania species in China by karyotype analysis. METHODS: DNA karyotypes of 16 Leishmania isolates in China were analyzed by pulsed field gel electrophoresis, and 5 different species of WHO reference Leishmania isolates were used in comparison. RESULTS: Chromosomal DNAs under 2 200 kb were resolved under pulsed field gel electrophoresis conditions. All the 16 isolates, even isolates in the same endemic area exhibited different chromosomal DNA banding patterns. The size variation in the chromosomal DNAs was evident particularly in chromosomes between 225 and 850 kb. Large-sized DNAs of more than 850 kb were more conserved than smaller-sized DNAs among 16 Leishmania isolates. DNA karyotype analysis showed: ① The 14 isolates belonging to L. infantum /donovani complex presented similar but different karyotype; the DNA banding patterns of those from the same endemic area were more similar than different areas, as seen in isolates of KS22, KS23, KS26, KS27, KS28, KS29, KS213 and KS216 in Kashi region, Xingjiang and in isolates of Liu and Xu in Karamay region, Xingjiang. The 771 isolates separated from sandfles in Bachu, Xingjiang exhibited 26 DNA bands, being more than the average 19- 21 DNA bands of other isolates separated from human patie ts. ② R12 and R 20 isolates having been previously named after L. turanica and L. gerbilli exhibited much more similar karyotypes, which were obviously different from other tested isolates and the five WHO reference isolates. CONCLUSION: ① Apparent in traspecific karyotype variations among L. donovani/infantum complex isolates in China, the more similarities in DNA banding patterns, the closerrelationship between the isolates. ② Eco-geographical isolation and sandfly vector have contributed to the heterogeneity of L.infantum /L.donovani complex in China. ③ The isolates R 12 and R20 tend to belong to one species/two closely-related species.

Key words: Leishmania, pulsed field gel electrophoresis, molecular karyotype

吴波;瞿靖琦. 中国利什曼原虫分离株分子核型分析[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(6): 425-431.

WuBo;QuJingqi. KARYOTYPE ANALYSIS OF LEISHMANIA ISOLATES IN CHINA *[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1998, 16(6): 425-431.

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