伯氏疟原虫对小鼠脾B细胞和自然杀伤细胞及其表面分子的影响

doi:10.12140/j.issn.1000-7423.2020.02.021

中国寄生虫学与寄生虫病杂志 ›› 2020, Vol. 38 ›› Issue (2): 255-258.doi: 10.12140/j.issn.1000-7423.2020.02.021

伯氏疟原虫对小鼠脾B细胞和自然杀伤细胞及其表面分子的影响

张杰森¹, 张梦欣¹, 韩孟伊¹, 陈冰霞¹, 李勇森¹, 金晨曦², 齐艳伟²^,^*()

1 广州医科大学第三临床学院,广州 511436
2 广州医科大学病原生物学与免疫学教研室,广州 511436

收稿日期:2019-09-26 出版日期:2020-04-30 发布日期:2020-05-11
通讯作者: 齐艳伟
作者简介:张杰森（1997-）,男,本科生,从事寄生虫免疫研究研究。E-mail： 411312320@qq.com
基金资助:
国家自然科学基金项目(81902087);广东省自然科学基金项目(2017A030310535);广东省自然科学基金项目(No. S201910570023);广东省大学生创新创业训练计划项目(S201910570027);广东省大学生创新创业训练计划项目(2018A036);广州医科大学大学生创新创业训练项目(2018A0024);广州医科大学第三临床学院大学生科研项目(2018A0020)

Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules

Jie-sen ZHANG¹, Meng-xin ZHANG¹, Meng-yi HAN¹, Bing-xia CHEN¹, Yong-sen LI¹, Chen-xi JIN², Yan-wei QI²^,^*()

1 The Third Clinical College, Guangzhou Medical University, Guangzhou 511436, China
2 Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou 511436, China

Received:2019-09-26 Online:2020-04-30 Published:2020-05-11
Contact: Yan-wei QI
Supported by:
Supported by the National Natural Science Foundation of China(81902087);Guangdong Natural Science Fund Project(2017A030310535);Guangdong Natural Science Fund Project(No. S201910570023);Guangdong University Student Innovation and Entrepreneurship Training Program(S201910570027);Guangzhou Medical University Student Innovation and Entrepreneurship Training Project(2018A036);Guangzhou Medical University Third Clinical College University Student Scientific Research Project(2018A0024);Guangzhou Medical University Third Clinical College University Scientific Research Project(2018A0020)

摘要/Abstract

摘要：

为探讨伯氏疟原虫对小鼠脾B细胞和自然杀伤（NK）细胞及其表面分子的影响,将10只雌性C57BL/6小鼠随机分为健康对照组和感染组,每组5只。感染组小鼠经尾静脉注射伯氏疟原虫（10 ⁶个/鼠）进行感染,健康对照组注射等量生理盐水。感染后6 d,取小鼠脾,制备单细胞悬液,采用流式细胞术检测小鼠B细胞和NK细胞的含量及其表面分子CD62L、CXCR3、CD69的表达水平。结果显示,感染组小鼠脾B细胞百分比含量为（79.2 ± 3.6）%,高于健康对照组的（54.3 ± 4.4）%（P < 0.01）;感染组脾NK细胞百分比为（2.2 ± 0.7）%,低于健康对照组的（4.6 ± 0.8)%（P < 0.01）。CD62L在感染组B细胞中的百分比为（77.7 ± 4.4）%,高于健康对照组的（72.8 ± 7.1）%,但二者差异无统计学意义（P > 0.05）;CD62L在感染组NK细胞中的百分比为（61.9 ± 4.8）%,低于健康对照组的（86.6 ± 4.2）%（P < 0.01）。CXCR3在感染组B细胞中的百分比为（4.1 ± 0.1）%,高于健康对照组的（3.0 ± 0.2）%（P < 0.01）;CXCR3在感染组NK细胞中的百分比为（2.1 ± 0.9）%,低于健康对照组的（2.3 ± 0.4）%,但二者差异无统计学意义（P > 0.05）。CD69在感染组B细胞和NK细胞中的百分比分别为（54.3 ± 4.7）%、（22.2 ± 1.6）%,均高于健康对照组的（1.9 ± 0.4）%、（1.3 ± 0.3）%（P < 0.01）。提示感染疟原虫的小鼠可能通过增加B细胞的数量以及上调其表面分子CXCR3和CD69及NK细胞表面分子CD69的表达水平发挥抗疟作用。

关键词: 伯氏疟原虫, 脾脏, 免疫细胞, 细胞表面分子

Abstract:

To investigate the affects of Plasmodium berghei infection on spleen B cells and NK cells as well as their surface molecules in mice, 10 female C57BL/6 mice were randomly assigned into control group and infection group, five mice each. The mice in the infection group were infected with P. berghei by injection through the tail vein (10 ⁶ parasites/mouse), while the control group received the same volume of saline. Six days after infection, spleens were collected to prepare single cell suspensions, and using flow cytometry to assay the amount of spleen B cells and NK cells and the expression of their surface molecules CD62L, CXCR3 and CD69. The results showed that the percentage of B cells in the infection group was (79.2 ± 3.6)%, significantly higher than that in the control group [(54.3 ± 4.4)%, P < 0.01]. The amount percentage of spleen NK cells in the infection group was (2.2 ± 0.7)%, significantly lower than that in the normal control group [(4.6 ± 0.8)%, P < 0.01]. The percentage of CD62L expression on B cells in the infection group was (77.7 ± 4.4)%, higher than that in the normal group (72.8 ± 7.1)%, but with no statistical significance (P > 0.05). The percentage of CD62L expression on NK cells in the infection group was (61.9 ± 4.8)%, which was significantly lower than that in the control group [(86.6 ± 4.2)%, P < 0.01]. The percentage of CXCR3 expression on B cells in the infection group was (4.1 ± 0.1)%, which was significantly higher than that in the control group [(3.0 ± 0.2) %, P < 0.01]. The percentage of CXCR3 expression on NK cells was (2.1 ± 0.9)%, which was lower than that of the control group [(2.3 ± 0.4)%], but with no statistical significance (P > 0.05). The percentages of CD69 expression on B cells and NK cells were (54.3 ± 4.7)% and (22.2 ± 1.6)%, respectively, both significantly higher than those in the control group [(1.9 ± 0.4)%, (1.3 ± 0.3)%, P < 0.01]. These results suggest that the mice infected with P. berghei may exert the affect against the infection through increasing B cell recruitment and up-regulating the expression of their surface molecules CXCR3 and CD69, and NK cell surface molecule CD69.

Key words: Plasmodium berghei, Spleen, Immune cells, Cell surface molecules

中图分类号:

R382.319

张杰森, 张梦欣, 韩孟伊, 陈冰霞, 李勇森, 金晨曦, 齐艳伟. 伯氏疟原虫对小鼠脾B细胞和自然杀伤细胞及其表面分子的影响[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(2): 255-258.

Jie-sen ZHANG, Meng-xin ZHANG, Meng-yi HAN, Bing-xia CHEN, Yong-sen LI, Chen-xi JIN, Yan-wei QI. Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2020, 38(2): 255-258.

图/表 2

参考文献 17

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伯氏疟原虫对小鼠脾B细胞和自然杀伤细胞及其表面分子的影响

Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules

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