关键词: 日本血吸虫, 总抗氧化能力, 蒿甲醚, 血红素

Abstract: 　Objective To study the effect of artemether (Art) on total antioxidant capacity (T-AOC) in adult Schisto-soma japonicum. Methods In vitro, the T-AOC was determined in five-week old worms incubated without or with Art and/ or hemin for 24 h, and the worms were continuously incubated for 96 h, then worm survival was assessed. In vivo, T-AOC was determined in worms freshly recovered from mice 6 - 24 h after treatment with Art 300 mg/kg. Results Throughout 96 h incubation no worms were killed by 50 μmol/L Art or 50 μmol/L hemin alone, but approximately 80% of them were killed by Art plus hemin. Addition of reduced glutathione and vitamin E could significantly block the tidal action of the combined treatment. No effect on T-AOC was seen in the worms exposed to Art or hemin alone for 24 h, but the combined treatment led to a pronounced T-AOC reduction in female worms in vitro. Such a drug effect on female worms was demonstrated in vivo. After female worms were exposed to Art for 6 - 24 h in vivo, their T-AOC was significantly reduced by 40% - 64%. However, no drug effect on male worms' T-AOC was observed in vivo and in vitro exposed to Art plus hemin. Conclusion Art-induced T-AOC reduction in female worms may sensitize them to lethal damages of endogenous and exogenous oxygen radicals.

Key words: Schistosoma japonicum, total antioxidant capacity, artemether, hemin