内蒙古黑鹳感染裂口凯塞玛吸虫的形态学与分子鉴定

doi:10.12140/j.issn.1000-7423.2025.04.022

中国寄生虫学与寄生虫病杂志 ›› 2025, Vol. 43 ›› Issue (4): 592-595.doi: 10.12140/j.issn.1000-7423.2025.04.022

内蒙古黑鹳感染裂口凯塞玛吸虫的形态学与分子鉴定

李健¹^,²^,³()(), 李奋奇², 高楠⁴, 柳强⁵, 周庆安⁶, 李春福², 胡薇²^,³^,^*()()

1 广西中医药大学基础医学院，广西南宁 530200
2 内蒙古大学生命科学学院，内蒙古呼和浩特 01002
3 复旦大学生命科学学院，上海 200438
4 呼和浩特市野生动植物保护协会，内蒙古呼和浩特 010021
5 呼和浩特市园林建设服务中心，内蒙古呼和浩特 010021
6 广西动物疫病预防控制中心，广西南宁 530200

收稿日期:2025-01-23 修回日期:2025-07-06 出版日期:2025-08-30 发布日期:2025-10-09
通讯作者: *胡薇（ORCID：0000-0002-4432-5400），女，博士，教授，从事人兽共患病原生物研究。E-mail：huw@fudan.edu.cn
作者简介:李健（ORCID：0000-0001-5907-0925），男，博士，副教授，从事寄生虫分类学研究。E-mail：leejianshin@163.com
基金资助:
国家科技基础条件平台国家寄生虫资源库项目(NPRC-2019-194-30);国家自然科学基金(U22A20526)

Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia

LI Jian¹^,²^,³()(), LI Fenqi², GAO Nan⁴, LIU Qiang⁵, ZHOU Qing’an⁶, LI Chunfu², HU Wei²^,³^,^*()()

1 College of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China
2 School of Life Sciences, Inner Mongolia University, Hohhot 010021, Inner Mongolia, China
3 School of Life Sciences, Fudan University, Shanghai 200438, China
4 Hohhot Wildlife and Plant Protection Association, Hohhot 010021, Inner Mongolia, China
5 Hohhot Landscaping Construction Service Center, Hohhot 010021, Inner Mongolia, China
6 Guangxi Center for Animal Disease Control and Prevention, Nanning 530200, Guangxi, China

Received:2025-01-23 Revised:2025-07-06 Online:2025-08-30 Published:2025-10-09
Contact: *E-mail: huw@fudan.edu.cn
Supported by:
National Parasitic Resources Center, the Ministry of Science and Technology Fund(NPRC-2019-194-30);National Natural Science Foundation of China(U22A20526)

摘要/Abstract

摘要：

2024年8月自内蒙古呼和浩特市大青山野生动物保护中心黑鹳的咽部与食道采集吸虫，取形态完整的成虫10条，盐酸卡红染色后形态观察。提取3条成虫基因组DNA，PCR扩增内转录间隔区2（ITS2）序列并测序，测序结果在GenBank数据库中进行BLAST比对，使用MEGA 11.0软件以邻接法构建系统进化树。在10只黑鹳咽部共采集到吸虫157条，感染强度为8~33条/只，平均约16条/只。吸虫体形呈叶片状、具分支状睾丸以及前位卵巢，形态符合裂口凯塞玛吸虫特征。PCR扩增和双向测序显示，获得的3条ITS2序列，长度均为425 bp，序列完全一致。提交GenBank，获得登录号：PQ899708。经BLAST比对发现，该序列与捷克黑鹳分离株在第365位碱基存在单一突变（C→T）。系统进化树显示，所获得的序列与GenBank中裂口凯塞玛吸虫聚为一支（自展值98%），表明其在种内具有单系性及相对稳定的遗传背景。

关键词: 裂口凯塞玛吸虫, 黑鹳, DNA内转录间隔区2, 种系发育分析

Abstract:

Trematodes were collected from the pharynxes and oesophagi of Ciconia nigra at the Daqingshan Wildlife Park in Hohhot, Inner Mongolia on August 2024. Ten morphologically intact adult worms were sampled and stained with hydrochloric carmine for morphological observation. Genomic DNA was extracted from three adult worms, and the internal transcribed spacer 2 (ITS2) gene was amplified using PCR assay and sequenced. ITS2 gene sequences were aligned using BLAST in GenBank and a phylogenetic tree was built using the software MEGA 11.0 with the neighbor-joining method. A total of 157 trematodes were collected from pharynxes of 10 C. nigra, with mean infection intensity of approximately 16 (range, 8 to 33) worms per bird. The trematodes appeared a leaf-like shape, with dendroidal testes and anterior ovary, which matched the morphological characteristics of Cathaemasia hians. PCR assay and paired-end sequencing yielded 3 ITS2 gene sequences, with 425 bp in length, which was identical to the ITS2 sequence of C. hians. The ITS2 sequence had been deposited in GenBank under accession number PQ899708. Sequence alignment showed that the ITS2 gene sequence had a single C→T substitution at position 365 with the Czech isolate of C. hians, and phylogenetic analysis revealed that the ITS2 gene sequence was clustered with the reference sequence of C. hians in GenBank into the same clade (98% bootstrap value), indicating that the ITS2 gene sequence had an intra-species monophyly and a relatively stable genetic background.

Key words: Cathaemasia hians, Ciconia nigra, Internal transcribed spacer 2, Phylogenetic analysis

中图分类号:

R383.2

李健, 李奋奇, 高楠, 柳强, 周庆安, 李春福, 胡薇. 内蒙古黑鹳感染裂口凯塞玛吸虫的形态学与分子鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(4): 592-595.

LI Jian, LI Fenqi, GAO Nan, LIU Qiang, ZHOU Qing’an, LI Chunfu, HU Wei. Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2025, 43(4): 592-595.

图/表 3

参考文献 8

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内蒙古黑鹳感染裂口凯塞玛吸虫的形态学与分子鉴定

Morphological and molecular identification of Cathaemasia hians in Ciconia nigra in Inner Mongolia

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