中缅边境盈江县微小按蚊嗜血习性调查

doi:10.12140/j.issn.1000-7423.2024.01.019

中国寄生虫学与寄生虫病杂志 ›› 2024, Vol. 42 ›› Issue (1): 125-128.doi: 10.12140/j.issn.1000-7423.2024.01.019

中缅边境盈江县微小按蚊嗜血习性调查

邹文健¹^,²(), 吴林波¹, 许翔¹, 唐烨榕¹, 孙晓东¹, 曾旭灿¹^,²^,^*()

1 云南省寄生虫病防治所，云南省热带传染病国际联合实验室，云南省虫媒传染病防控研究重点实验室，云南省虫媒传染病防控关键技术创新团队普洱 665000
2 昆明医科大学，云南昆明 650500

收稿日期:2023-08-17 修回日期:2023-09-11 出版日期:2024-02-28 发布日期:2024-03-12
通讯作者: *曾旭灿（1983—），男，本科，副主任医师，从事媒介控制工作。E-mail：531044248@qq.com
作者简介:邹文健（1998—），男，本科，医师，从事疾病控制预防工作。E-mail：axxdzwj@163.com
基金资助:
国家自然科学基金(81960374);云南省重点研发计划项目(202103AQ100001);澜湄合作专项基金项目(2020399)

Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border

ZOU Wenjian¹^,²(), WU Linbo¹, XU Xiang¹, TANG Yerong¹, SUN Xiaodong¹, ZENG Xucan¹^,²^,^*()

1 Yunnan Institute of Parasitic Diseases, Yunnan International Joint Laboratory of Tropical Infectious Diseases, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention, Pu’er 665000, China
2 Kunming Medical University, Kunming 650500, Yunnan, China

Received:2023-08-17 Revised:2023-09-11 Online:2024-02-28 Published:2024-03-12
Contact: *E-mail: 531044248@qq.com
Supported by:
National Natural Science Foundation of China(81960374);Key Research and Development Programs in Yunnan Province(202103AQ100001);Specialized Fund for Lancang Mekong Cooperation(2020399)

摘要/Abstract

摘要：

为了解中缅边境地区疟疾主要传播媒介微小按蚊的嗜血习性，2021年5—7月在云南省盈江县那邦镇采用紫外诱蚊灯法诱捕蚊虫。捕获的蚊虫进行形态学鉴定并记录雌蚊的吸血情况（腹部鲜红色的为饱血雌蚊），提取初步鉴定为微小按蚊的单只蚊虫基因组DNA，PCR扩增细胞色素C氧化酶亚基1（cox1）基因，阳性产物测序后进行序列比对确定蚊种。多重PCR扩增核糖体DNA第二内转录间隔区（ITS2）序列鉴定亚种，扩增线粒体DNA细胞色素b（cytb）基因鉴定饱血雌蚊的胃血源。共捕获蚊虫3 419只，经形态学初步鉴定为微小按蚊的有303只，其中245只蚊的DNA经PCR扩增出500 bp的cox1阳性条带，其序列与微小按蚊（GenBank登录号：GQ259177.1）的序列一致性均在99%以上。ITS2序列多重PCR扩增结果显示，245只微小按蚊均为A型。20份饱血微小按蚊cytb基因的多重PCR扩增结果显示，4份与人血阳性对照一致、14份与猪血阳性对照一致、2份与牛血阳性对照一致，人血指数、猪血指数和牛血指数分别为0.2（4/20）、0.7（14/20）和0.1（2/20）。本研究显示，云南省盈江县优势按蚊种群为微小按蚊，其亚种类型均为A型；嗜血习性为人畜兼食，疟疾传播风险较高。

关键词: 微小按蚊, 嗜血习性, 中缅边境

Abstract:

To understand the host-feeding habits of Anopheles minimus, the main malaria vector at China-Myanmar border areas, mosquitoes were captured from May to July 2021 in Nabang Town, Yingjiang County, Yunnan Province by the ultraviolet mosquito trap method. The captured mosquitoes were identified by morphology. The blood-sucking status of female mosquitoes was recorded: the female mosquitos with scarlet abdomens were regarded as blood-filled mosquitos. The genomic DNA of the mosquitoes identified as An. minimus were extracted, and the cytochrome C oxidase subunit 1 gene (cox1) were amplified by PCR. The sequence comparison of the positive products was performed to determine the mosquito species. Subspecies identification was based on multiplex PCR amplification of the second internal transcribed spacer (ITS2) sequence of ribosomal DNA. The blood sources identification of blood-filled female mosquitos was based on multiplex PCR amplification of cytochrome b (cytb) of mitochondrial DNA. A total of 3 419 mosquitoes were captured and 303 An. minimus were identified by morphology initially. A total of 245 mosquitoes were cox1 positive by PCR, and the sequences were over 99% identical to An. minimus (GenBank accession number: GQ259177.1). ITS2 multiplex PCR amplification results showed that all 245 An. minimus mosquitos were type A. Cytb multiplex PCR amplification results of 20 blood-filled female mosquitos showed that the blood sources of 4 mosquitos were from humans, 14 were from pigs and 2 were from cows. The human, pig, and cow blood index were 0.2 (4/20), 0.7 (14/20) and 0.1 (2/20), respectively. This study showed that the dominant Anopheles population in Yingjiang County, Yunnan Province were An. minimus, whose subspecies types were all type A. Their preferred host includes both animals and humans. The risk of malaria transmission was high.

Key words: Anopheles minimus, Host-feeding habits, China-Myanmar border

中图分类号:

384.111

邹文健, 吴林波, 许翔, 唐烨榕, 孙晓东, 曾旭灿. 中缅边境盈江县微小按蚊嗜血习性调查[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 125-128.

ZOU Wenjian, WU Linbo, XU Xiang, TANG Yerong, SUN Xiaodong, ZENG Xucan. Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2024, 42(1): 125-128.

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引物名称	序列（5′→3′）	产物长度/bp
蚊种鉴定
cox1-F	GGAGGATTTGGAAATTGATTGATTAGTTC	500
cox1-R	CCCGGTAAAATTAAAATATAAACTTC
微小按蚊亚种鉴定
ITS2-F	ATCACTCGGCTCATGGATCG
ITS2-A-R	GGGCGCCATGTAGTTAGAGTTG	184
ITS2-C-R	GGTTGCCCACTCAATACGGGTG	509
蚊胃血源鉴定
cytb-hum-F	GGCTTACTTCTCTTCATTCTCTCCT	334
cytb-pig-F	CCTCGCAGCCGTACATCTC	453
cytb-cow-F	CATCGGCACAAATTTAGTCG	561
cytb-dog-F	GGAATTGTACTATTATTCGCAACCAT	680
cytb-R	GGTTGTCCTCCAATTCATGTTA

中缅边境盈江县微小按蚊嗜血习性调查

Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border

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