中缅边境盈江县微小按蚊嗜血习性调查

doi:10.12140/j.issn.1000-7423.2024.01.019

中国寄生虫学与寄生虫病杂志 ›› 2024, Vol. 42 ›› Issue (1): 125-128.doi: 10.12140/j.issn.1000-7423.2024.01.019

中缅边境盈江县微小按蚊嗜血习性调查

邹文健¹^,²(), 吴林波¹, 许翔¹, 唐烨榕¹, 孙晓东¹, 曾旭灿¹^,²^,^*()

1 云南省寄生虫病防治所，云南省热带传染病国际联合实验室，云南省虫媒传染病防控研究重点实验室，云南省虫媒传染病防控关键技术创新团队普洱 665000
2 昆明医科大学，云南昆明 650500

收稿日期:2023-08-17 修回日期:2023-09-11 出版日期:2024-02-28 发布日期:2024-03-12
通讯作者: *曾旭灿（1983—），男，本科，副主任医师，从事媒介控制工作。E-mail：531044248@qq.com
作者简介:邹文健（1998—），男，本科，医师，从事疾病控制预防工作。E-mail：axxdzwj@163.com
基金资助:
国家自然科学基金(81960374);云南省重点研发计划项目(202103AQ100001);澜湄合作专项基金项目(2020399)

Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border

ZOU Wenjian¹^,²(), WU Linbo¹, XU Xiang¹, TANG Yerong¹, SUN Xiaodong¹, ZENG Xucan¹^,²^,^*()

1 Yunnan Institute of Parasitic Diseases, Yunnan International Joint Laboratory of Tropical Infectious Diseases, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention, Pu’er 665000, China
2 Kunming Medical University, Kunming 650500, Yunnan, China

Received:2023-08-17 Revised:2023-09-11 Online:2024-02-28 Published:2024-03-12
Contact: *E-mail: 531044248@qq.com
Supported by:
National Natural Science Foundation of China(81960374);Key Research and Development Programs in Yunnan Province(202103AQ100001);Specialized Fund for Lancang Mekong Cooperation(2020399)

摘要/Abstract

摘要：

为了解中缅边境地区疟疾主要传播媒介微小按蚊的嗜血习性，2021年5—7月在云南省盈江县那邦镇采用紫外诱蚊灯法诱捕蚊虫。捕获的蚊虫进行形态学鉴定并记录雌蚊的吸血情况（腹部鲜红色的为饱血雌蚊），提取初步鉴定为微小按蚊的单只蚊虫基因组DNA，PCR扩增细胞色素C氧化酶亚基1（cox1）基因，阳性产物测序后进行序列比对确定蚊种。多重PCR扩增核糖体DNA第二内转录间隔区（ITS2）序列鉴定亚种，扩增线粒体DNA细胞色素b（cytb）基因鉴定饱血雌蚊的胃血源。共捕获蚊虫3 419只，经形态学初步鉴定为微小按蚊的有303只，其中245只蚊的DNA经PCR扩增出500 bp的cox1阳性条带，其序列与微小按蚊（GenBank登录号：GQ259177.1）的序列一致性均在99%以上。ITS2序列多重PCR扩增结果显示，245只微小按蚊均为A型。20份饱血微小按蚊cytb基因的多重PCR扩增结果显示，4份与人血阳性对照一致、14份与猪血阳性对照一致、2份与牛血阳性对照一致，人血指数、猪血指数和牛血指数分别为0.2（4/20）、0.7（14/20）和0.1（2/20）。本研究显示，云南省盈江县优势按蚊种群为微小按蚊，其亚种类型均为A型；嗜血习性为人畜兼食，疟疾传播风险较高。

关键词: 微小按蚊, 嗜血习性, 中缅边境

Abstract:

To understand the host-feeding habits of Anopheles minimus, the main malaria vector at China-Myanmar border areas, mosquitoes were captured from May to July 2021 in Nabang Town, Yingjiang County, Yunnan Province by the ultraviolet mosquito trap method. The captured mosquitoes were identified by morphology. The blood-sucking status of female mosquitoes was recorded: the female mosquitos with scarlet abdomens were regarded as blood-filled mosquitos. The genomic DNA of the mosquitoes identified as An. minimus were extracted, and the cytochrome C oxidase subunit 1 gene (cox1) were amplified by PCR. The sequence comparison of the positive products was performed to determine the mosquito species. Subspecies identification was based on multiplex PCR amplification of the second internal transcribed spacer (ITS2) sequence of ribosomal DNA. The blood sources identification of blood-filled female mosquitos was based on multiplex PCR amplification of cytochrome b (cytb) of mitochondrial DNA. A total of 3 419 mosquitoes were captured and 303 An. minimus were identified by morphology initially. A total of 245 mosquitoes were cox1 positive by PCR, and the sequences were over 99% identical to An. minimus (GenBank accession number: GQ259177.1). ITS2 multiplex PCR amplification results showed that all 245 An. minimus mosquitos were type A. Cytb multiplex PCR amplification results of 20 blood-filled female mosquitos showed that the blood sources of 4 mosquitos were from humans, 14 were from pigs and 2 were from cows. The human, pig, and cow blood index were 0.2 (4/20), 0.7 (14/20) and 0.1 (2/20), respectively. This study showed that the dominant Anopheles population in Yingjiang County, Yunnan Province were An. minimus, whose subspecies types were all type A. Their preferred host includes both animals and humans. The risk of malaria transmission was high.

Key words: Anopheles minimus, Host-feeding habits, China-Myanmar border

中图分类号:

384.111

邹文健, 吴林波, 许翔, 唐烨榕, 孙晓东, 曾旭灿. 中缅边境盈江县微小按蚊嗜血习性调查[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 125-128.

ZOU Wenjian, WU Linbo, XU Xiang, TANG Yerong, SUN Xiaodong, ZENG Xucan. Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2024, 42(1): 125-128.

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参考文献 20

[1]	Chen TM, Zhou HN, Luo CH, et al. Composition of anopheline larvae in the China-Myanmar border region in Yingjiang County of Yunnan Province[J]. Chin J Parasitol Parasit Dis, 2018, 36(2): 112-118. (in Chinese)
	(陈田木, 周红宁, 罗春海, 等. 云南省盈江县边境地区按蚊孳生地幼蚊种类构成分析[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(2): 112-118.)
[2]	Zeng XC, Sun XD, Li JX, et al. Assessment of malaria control consultation and service posts in Yunnan, P. R. China[J]. Infect Dis Poverty, 2016, 5: 102. doi: 10.1186/s40249-016-0185-y
[3]	Duan LY. Dictionary of political regions of PRC, Yunnan Province[M]. Beijing: China Social Press, 2016:2612-2613. (in Chinese)
	(段丽元. 中华人民共和国政区大典云南省卷[M]. 北京: 中国社会出版社, 2016: 2612-2613.)
[4]	Wu LB, Xu X, Guo XL, et al. Investigation of the mosquito invasion of different types of housing in the China-Myanmar border area[J]. J Pathog Biol, 2021, 16(7): 853-855, 858. (in Chinese)
	(吴林波, 许翔, 郭小连, 等. 中缅边境地区不同建筑类型房屋蚊虫入侵情况调查[J]. 中国病原生物学杂志, 2021, 16(7): 853-855, 858.)
[5]	Dong XS. Keys taxonmia to the Anopheles of China[M]. Kunming: Yunnan Science and Technology Press, 2014: 30-41. (in Chinese)
	(董学书. 中国按蚊分类检索[M]. 昆明: 云南科技出版社, 2014: 30-41.)
[6]	Yang MN, Ma YJ. Identification of mosquito blood meals using multiplex polymerase chain reaction[J]. J Pathog Biol, 2008, 3(11): 815-817, 814. (in Chinese)
	(杨曼尼, 马雅军. 应用多重PCR法鉴定蚊胃血血源的研究[J]. 中国病原生物学杂志, 2008, 3(11): 815-817, 814.)
[7]	Gao B, Wang YP, Xu Q, et al. COI sequences analysis for three kinds of mosquitoes at Fujian port[J]. Chin J Front Health Quar, 2010, 33(6): 398-401. (in Chinese)
	(高博, 王宇平, 许卿, 等. 福建口岸三蚊种COI基因序列分析[J]. 中国国境卫生检疫杂志, 2010, 33(6): 398-401.)
[8]	Phuc HK, Ball AJ, Son L, et al. Multiplex PCR assay for malaria vector Anopheles minimus and four related species in the Myzomyia series from Southeast Asia[J]. Med Vet Entomol, 2003, 17(4): 423-428. pmid: 14651657
[9]	Yu G, Yan GY, Zhang NX, et al. The Anopheles community and the role of Anopheles minimus on malaria transmission on the China-Myanmar border[J]. Parasit Vectors, 2013, 6(1): 264. doi: 10.1186/1756-3305-6-264
[10]	Chen B, Harbach RE, Butlin RK. Molecular and morphological studies on the Anopheles minimus group of mosquitoes in Southern China: taxonomic review, distribution and malaria vector status[J]. Med Vet Entomol, 2002, 16(3): 253-265. pmid: 12243226
[11]	Garros C, Van Nguyen C, Trung HD, et al. Distribution of Anopheles in Vietnam, with particular attention to malaria vectors of the Anopheles minimus complex[J]. Malar J, 2008, 7: 11. doi: 10.1186/1475-2875-7-11
[12]	Foley DH, Rueda LM, Peterson AT, et al. Potential distribution of two species in the medically important Anopheles minimus complex (Diptera ∶ Culicidae)[J]. J Med Entomol, 2008, 45(5): 852-860. doi: 10.1093/jmedent/45.5.852
[13]	Zheng B, Tang LH, Ma YJ, et al. Population genetics study on Anopheles minimus in Yunnan Province by SSR-PCR[J]. J Trop Med, 2007, 7(6): 529-532, 535. (in Chinese)
	(郑彬, 汤林华, 马雅军, 等. 微卫星锚定PCR技术研究云南微小按蚊群体遗传结构[J]. 热带医学杂志, 2007, 7(6): 529-532, 535.)
[14]	Zhou XJ, Shi WQ, Zhang Y, et al. Distribution of Anopheles minimus and its role in malaria transmission in the Kachin Region of Myanmar[J]. J Pathog Biol, 2010, 5(8): 578-580, 584. (in Chinese)
	(周晓俊, 施文琦, 张仪, 等. 缅甸克钦地区媒介微小按蚊分布和传疟作用[J]. 中国病原生物学杂志, 2010, 5(8): 578-580, 584.)
[15]	Liu YB, Cao J, Zhou HY, et al. Assay of human blood index of anopheline mosquito by polymerase chain reaction[J]. Chin J Vector Biol Control, 2009, 20(2): 108-110. (in Chinese)
	(刘耀宝, 曹俊, 周华云, 等. 用PCR技术测定按蚊人血指数的研究[J]. 中国媒介生物学及控制杂志, 2009, 20(2): 108-110.)
[16]	Gao BH. Studying on blood meal habits of Anopheles in Yunnan from 1976 to 1986[J]. Chin J Pest Control, 2002, 18(9): 486-488. (in Chinese)
	(高白荷. 云南省1976—1986年按蚊嗜血习性调查研究[J]. 医学动物防制, 2002, 18(9): 486-488.)
[17]	Wang PY, Bi Y. Identification of host source of blood meals on four Anopheles species in Yunnan[J]. Chin J Vector Biol Control, 1995, 6(5): 332-333. (in Chinese)
	(王丕玉, 毕艳. 云南四种按蚊胃血血源鉴定研究报告[J]. 中国媒介生物学及控制杂志, 1995, 6(5): 332-333.)
[18]	Guo SH, Zhou SS. Identification methods of the blood meals in mosquitoes[J]. Chin J Zoonoses, 2012, 4(28): 380-388. (in Chinese)
	(郭绍华, 周水森. 蚊虫胃血血源鉴定方法[J]. 中国人兽共患病学报, 2012. 4(28): 380-388.)
[19]	Zhang JW, Jiang JY, Wang XZ, et al. Identification of mammalian blood meals in anopheline mosquitoes from four counties of Yunnan Province by multiple PCR[J]. Chin J Parasitol Parasit Dis, 2014, 32(1): 76-77. (in Chinese)
	(张井巍, 姜进勇, 王学忠, 等. 应用多重PCR法鉴定分析云南4县按蚊胃血源[J]. 中国寄生虫学与寄生虫病杂志, 2014, 32(1): 76-77.)
[20]	Zhang SS, Zhou SS, Zhou ZB, et al. Investigation on population density and bionomics of Anopheles minimus in China-Myanmar border[J]. Chin J Vector Biol Control, 2017, 28(3): 216-219, 254. (in Chinese)
	(张少森, 周水森, 周正斌, 等. 中国-缅甸边境地区微小按蚊种群密度及其生态习性调查[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 216-219, 254.) doi: 10.11853/j.issn.1003.8280.2017.03.005

引物名称	序列（5′→3′）	产物长度/bp
蚊种鉴定
cox1-F	GGAGGATTTGGAAATTGATTGATTAGTTC	500
cox1-R	CCCGGTAAAATTAAAATATAAACTTC
微小按蚊亚种鉴定
ITS2-F	ATCACTCGGCTCATGGATCG
ITS2-A-R	GGGCGCCATGTAGTTAGAGTTG	184
ITS2-C-R	GGTTGCCCACTCAATACGGGTG	509
蚊胃血源鉴定
cytb-hum-F	GGCTTACTTCTCTTCATTCTCTCCT	334
cytb-pig-F	CCTCGCAGCCGTACATCTC	453
cytb-cow-F	CATCGGCACAAATTTAGTCG	561
cytb-dog-F	GGAATTGTACTATTATTCGCAACCAT	680
cytb-R	GGTTGTCCTCCAATTCATGTTA

中缅边境盈江县微小按蚊嗜血习性调查

Investigation on host-feeding habits of Anopheles minimus in Yingjiang County at the China-Myanmar border

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