河南省洛阳市并殖吸虫病疫源地流行病学调查

doi:10.12140/j.issn.1000-7423.2020.05.023

中国寄生虫学与寄生虫病杂志 ›› 2020, Vol. 38 ›› Issue (5): 664-666.doi: 10.12140/j.issn.1000-7423.2020.05.023

河南省洛阳市并殖吸虫病疫源地流行病学调查

李云霞¹(), 艾琳², 邓艳³, 陈伟奇³, 朱鑫¹, 杨治国¹, 李克伟¹, 孙毅莹¹

1 洛阳市疾病预防控制中心,洛阳 471023
2 中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025
3 河南省疾病预防控制中心,郑州 450016

收稿日期:2020-03-21 出版日期:2020-10-30 发布日期:2020-11-12
作者简介:李云霞（1972-）,女,本科,副主任医师,从事并殖吸虫病研究。E-mail:lyx1205@126.com

Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province

LI Yun-xia¹(), AI Lin², DENG Yan³, CHEN Wei-qi³, ZHU Xin¹, YANG Zhi-guo¹, LI Ke-wei¹, SUN Yi-ying¹

1 Luoyang Center for Disease Control and Prevention, Luoyang 471023, China
2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Center of Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
3 Luoyang Center for Disease Control and Prevention, Zhengzhou 450016, China

Received:2020-03-21 Online:2020-10-30 Published:2020-11-12

摘要/Abstract

摘要：

对河南省洛阳市溪蟹的并殖吸虫感染情况进行调查,确认洛阳市并殖吸虫病的疫源地。于2013年8月至2019年10月陆续在洛阳9县（市）监测点采集并殖吸虫第二中间宿主溪蟹,实验室检测溪蟹并殖吸虫感染情况。囊蚴采用PCR扩增内转录间隔区2（ITS2）序列、线粒体细胞色素氧化酶1（CO1）基因进行鉴定。共捕获560只溪蟹,宜阳县、洛宁县、新安县、嵩县和栾川县发现并殖吸虫囊蚴,经形态学和PCR鉴定为斯氏并殖吸虫囊蚴。宜阳县溪蟹感染率和感染度最高,分别为58.33%和11.54个囊蚴/只,新安县、嵩县、洛宁县和栾川县溪蟹的感染率和感染度依次为25.93%和0.33个囊蚴/只、19.64%和0.23个囊蚴/只、11.96%和0.12个囊蚴/只、6.17%和0.06个囊蚴/只。PCR结果显示,宜阳县、新安县、嵩县、洛宁县和栾川县溪蟹分离出的囊蚴样品扩增出的ITS2长度约500 bp,与斯氏并殖吸虫（GenBank登录号为KX129924.1）的相似性为98%~100%。提示宜阳县、洛宁县、新安县、栾川县和嵩县均为并殖吸虫病疫源地,其中宜阳县属于中度流行区,新安县、洛宁县、栾川县、嵩县为轻度流行区。

关键词: 并殖吸虫, 溪蟹, 疫源地, 调查, 洛阳

Abstract:

To investigate the status of Paragonimus spp. infection in freshwater crabs in Luoyang City of Henan Province, and identify the epidemic focus of paragonimiasis. Freshwater crabs as the second intermediate host were collected from surveillance sites in 9 counties(cities) of Luoyang City during the time from August 2013 to October 2019. Paragonimus spp. infection in the crabs was detected in laboratory settings. Paragonimus spp. metacercariae were identified by PCR amplification of the sequence of internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase subunit 1(CO1). A total of 560 freshwater crabs were captured, and Paragonimus metacercariae were found in the crabs from Yiyang County, Luoning County, Xin’an County, Song County, and Luanchuan County, and identified as the species P. skrjabini by morphological observation and PCR amplification. The infection rate and infectivity were found highest in the crabs collected from Yiyang County (58.33%, 11.54 metacercariae/crab), followed by Xin’an County (25.93%, 0.33 metacercariae/crab), Song County (19.64%, 0.23 metacercariae/crab), Luoning County (11.96%, 0.12 metacercariae/crab), and Luanchuan County (6.17%, 0.06 metacercariae/crab). Results of PCR showed that the ITS2 amplification product has a sequence length of 500 bp from the metacercariae isolated from the crabs of Yiyang County, Xin’an County, Songxian County, Luoning County and Luanchuan County, with 98%-100% homology to P. skrjabini (GenBank accession No. KX129924.1). It was recognized that five counties including Yiyang County, Luoning County, Xin’an County, Luanchuan County, and Song County were identified to be the epidemic foci of paragonimiasis among them, Yiyang County was a moderate endemic area, while the others being mild endemic regions.

Key words: Paragonimus, Freshwater crabs, Endemic focus, Investigation, Luoyang

中图分类号:

R532.22

李云霞, 艾琳, 邓艳, 陈伟奇, 朱鑫, 杨治国, 李克伟, 孙毅莹. 河南省洛阳市并殖吸虫病疫源地流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5): 664-666.

LI Yun-xia, AI Lin, DENG Yan, CHEN Wei-qi, ZHU Xin, YANG Zhi-guo, LI Ke-wei, SUN Yi-ying. Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2020, 38(5): 664-666.

图/表 2

参考文献 16

[1]	Blair D. Paragonimiasis[J]. Adv Exp Med Biol, 2019,1154:105-138.
[2]	Zhu XP, Su C. Human parasitology[M]. Beijing: People’s Medical Publishing House, 2013. (in Chinese)
[2]	( 诸欣平, 苏川. 人体寄生虫学[M]. 北京: 人民卫生出版社, 2013.)
[3]	Oh MY, Chu A, Park JH, et al. Simultaneous Paragonimus infection involving the breast and lung: A case report[J]. World J Clin Cases, 2019,7(24):4292-4298.
[4]	Yoshida A, Doanh PN, Maruyama H. Paragonimus and paragonimiasis in Asia: an update[J]. Acta Trop, 2019,199:105074.
[5]	World Health Organization. Investing to overcome the global impact of neglected tropical disease: third WHO report on neglected tropical disease[R]. WHO: Geneva, 2015.
[6]	Wang LD. A national survey on current status of the important parasitic diseases in human population[M]. Beijing: People’s Medical Publishing House, 2008. (in Chinese)
[6]	( 王陇德. 全国人体重要寄生虫病现状调查[M]. 北京: 人民卫生出版社, 2008.)
[7]	Zhou XN. Report on the national survey of important human parasitic diseases in China in 2015[M]. Beijing: People’s Medical Publishing House, 2018. (in Chinese)
[7]	( 周晓农. 2015年全国人体重点寄生虫病现状调查报告[M]. 北京: 人民卫生出版社, 2018.)
[8]	Chen GX, Chang J, Zhang XQ, et al. A preliminary investigation of freshwater crabs as the second intermediate host of Paragonimus in Henan Province[J]. Chin J Zool, 1982(4):1-3. (in Chinese)
[8]	( 陈国孝, 常江, 张秀清, 等. 河南省肺吸虫第二中间宿主淡水蟹类的初步调查[J]. 动物学杂志, 1982(4):1-3.)
[9]	Guo ZY, Liu Y. 36 patients with paragonimiasis investigation report[J]. Henan J Prev Med, 2013,24(3):217-219. (in Chinese)
[9]	( 郭正印, 刘英. 36例肺吸虫病调查报告[J]. 河南预防医学杂志, 2013,24(3):217-219.)
[10]	Wei HW, Cheng YZ, Zeng Y, et al. A survey of the new epidemic area of Paragonimus skriabini in Zhenghe, northern Fujian[J]. J Trop Med, 2010,10(1):83-84, 89. (in Chinese)
[10]	( 魏焕旺, 程由注, 曾永平, 等. 福建北部政和县斯氏并殖吸虫病新疫区调查[J]. 热带医学杂志, 2010,10(1):83-84, 89.)
[11]	Dai AY, Song YZ. Description of new species of freshwater crabs as intermediate hosts of Paragonimus and other flukes in China[J]. J zool, 1975,21(3):261-262. (in Chinese)
[11]	( 戴爱云, 宋玉枝. 我国肺吸虫及他种吸虫中间宿主——淡水蟹类新种描述[J]. 动物学报, 1975,21(3):261-262.)
[12]	Sheng YP, Zhang YJ. Preliminary observation on the biology and protein bands of Paragonimus sanping[J]. Chin J Parasitol Parasit Dis, 1987,5(4):278-280. (in Chinese)
[12]	( 沈一平, 张耀娟. 三平正并殖吸虫囊蚴的生物学与蛋白质区带初步观察[J]. 中国寄生虫学与寄生虫病杂志, 1987,5(4):278-280.)
[13]	Wu ZD, Huang Y, Song LG. New challenge for human parasitic disease control in China: food-borne parasitic disease control[J]. China Trop Med, 2019,19(1):1-3. (in Chinese)
[13]	( 吴忠道, 黄艳, 宋兰桂. 我国人体寄生虫病防治的新挑战: 食源性寄生虫病的防治[J]. 中国热带医学, 2019,19(1):1-3.)
[14]	Chen JX. Food-borne parasitic disease[M]. Beijing: People’s Medical Publishing House, 2009. (in Chinese)
[14]	( 陈家旭. 食源性寄生虫病[M]. 北京: 人民卫生出版社, 2009.)
[15]	Ma YX, Shang LY, Xu BL. Analysis and prevention of parasitic diseases in Henan Province[M]. Zhengzhou: Henan People’s Publishing House, 2014. (in Chinese)
[15]	( 马云祥, 尚乐园, 许汴利. 河南省寄生虫病防治与研究[M]. 郑州: 河南人民出版社, 2014.)
[16]	Chen W, Deng Y, Zhang YL, et al. A case of group infections with Paraginimus species in Henan, Central China[J]. Acta Trop, 2020,202:105111.

河南省洛阳市并殖吸虫病疫源地流行病学调查

Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province

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