广西宾阳县和灵山县水源隐孢子虫和蓝氏贾第鞭毛虫污染调查

中国寄生虫学与寄生虫病杂志 ›› 2018, Vol. 36 ›› Issue (6): 597-601.

广西宾阳县和灵山县水源隐孢子虫和蓝氏贾第鞭毛虫污染调查

曹胜魁¹, 姜岩岩¹, 袁忠英¹, 徐梦¹, 曹建平¹, 杨益超², 沈玉娟^1,^*()

1 中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025
2 广西壮族自治区疾病预防控制中心,南宁 530028

收稿日期:2018-01-30 出版日期:2018-12-30 发布日期:2019-01-08
通讯作者: 沈玉娟
基金资助:
国家公益性卫生行业科研专项(No. 201502021);上海市卫生和计划生育委员会青年基金(No. 20164Y0085);上海市公共卫生三年行动计划第四轮（No. 15GWZK0101）

Contamination of Cryptosporidium and Giardia lamblia in water from Binyang County and Lingshan County in Guangxi

Sheng-kui CAO¹, Yan-yan JIANG¹, Zhong-ying YUAN¹, Meng XU¹, Jian-ping CAO¹, Yi-chao YANG², Yu-juan SHEN^1,^*()

1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
2 Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China

Received:2018-01-30 Online:2018-12-30 Published:2019-01-08
Contact: Yu-juan SHEN
Supported by:
Supported by the Chinese Special Program for Scientific Research of Public Health (No. 201502021), the Shanghai Municipal Commission of Health and Family Planning (No. 20164Y0085)and the Fourth Round of Three-Year Public Health Action Plan of Shanghai (No. 15GWZK0101)

摘要/Abstract

摘要：

目的了解广西壮族自治区宾阳县和灵山县水源中隐孢子虫和蓝氏贾第鞭毛虫（简称贾第虫）的污染情况。方法利用质控标样检验碳酸钙絮凝和膜过滤富集隐孢子虫卵囊、贾第虫包囊的回收率,并与我国的国标方法比较。2016、2017年6-9月,采集广西壮族自治区宾阳县（自来水厂A、B、C、D和E）和灵山县（自来水厂F、G、H、I和J）10个自来水厂的进水和出厂水水样,应用碳酸钙絮凝法富集进水中隐孢子虫卵囊和贾第虫包囊,膜过滤法富集出厂水中隐孢子虫卵囊和贾第虫包囊;富集后用磁珠分选和荧光染色检测隐孢子虫卵囊和贾第虫包囊,计算水样中隐孢子虫卵囊和贾第虫包囊的密度。结果碳酸钙絮凝法富集质控标样隐孢子虫卵囊和贾第虫包囊的回收率分别为48.5%和52.0%,膜过滤法为35.0%和36.5%,国标方法为15.0%和16.0%,碳酸钙絮凝法、膜过滤法的回收率均高于我国国标方法,差异具有统计学意义（碳酸钙絮凝法：χ² = 26.007、28.877,均P < 0.01;膜过滤法：χ² = 8.167、11.019,均P < 0.01）。10个自来水厂中,每个自来水厂采集进水和出厂水水样各2份。检测结果显示：自来水厂A的进水、B的进水和出厂水、C和F的出厂水中检出隐孢子虫卵囊,密度分别为4.0、10.0、0.6、2.9、5.1个/10 L;自来水厂F的出厂水中检出贾第虫包囊,密度为9.3个/10 L。其余自来水厂的进水和出厂水均未检出隐孢子虫和贾第虫。结论广西壮族自治区宾阳县和灵山县部分自来水厂的进水或出厂水中存在隐孢子虫或贾第虫污染,需加强农村地区饮用水隐孢子虫和贾第虫的监测。

关键词: 隐孢子虫, 蓝氏贾第鞭毛虫, 碳酸钙絮凝, 膜过滤, 富集, 饮用水

Abstract:

ObjectiveTo investigate the contamination of Cryptosporidium and Giardia in water from Binyang County and Lingshan County in Guangxi Zhuang Autonomous Region. Methods Standard quality-control samples were used to compare the recovery rate of Cryptosporidium oocyst and Giardia cyst by calcium carbonate flocculation method and that by membrane filtration, with reference to the National Standard Method. In addition, water samples from 10 tap water suppliers in Binyang (suppliers A, B, C, D and E) and Lingshan (suppliers F, G, H, I and J) counties were collected in 2016 and 2017, including water flowing into and out from the suppliers. Calcium carbonate flocculation method was used to concentrate Cryptosporidium oocysts and Giardia cysts in water flowing into the suppliers, while membrane filtration was used to concentrate Cryptosporidium oocysts and Giardia cysts in water flowing out from the suppliers. Immunomagnetic separation and immunofluorescence staining were then performed to detect the two types of parasites in water, and their densities were calculated. Results The recovery rates of Cryptosporidium oocyst and Giardia cyst in standard quality control samples using calcium carbonate flocculation were 48.5% and 52.0%, respectively, and those by membrane filtration were 35.0% and 36.5%, both performed better than the National Standard Method(15.0% and 16.0%) (calcium carbonate flocculation vs. Standard Method:χ² = 26.007, 28.877, P < 0.01; membrane filtration vs. Standard Method:χ² = 8.167, 11.019, P < 0.01). In addition, 10 tap water suppliers were selected, from each of which 2 water samples flowing into and 2 water samples flowing out were collected. The densities of Cryptosporidium oocysts in the water sample flowing into supplier A, water samples flowing into and out from supplier B, and water samples flowing out from suppliers C and F were 4.0/10 L, 10.0/10 L, 0.6/10 L, 2.9/10 L and 5.1/10 L, respectively. The density of Giardia cysts in water sample flowing out from suppliers F was 9.3/10 L. No Cryptosporidium or Giardia was detected in other water samples. Conclusion There are certain contaminations of Cryptosporidium and Giardia in water flowing into or out from water suppliers in Binyang and Lingshan counties. Monitoring on the contamination in rural areas should be strengthened.

Key words: Cryptosporidium, Giardia lamblia, Calcium carbonate flocculation, Membrane filtration, Concentration, Water

中图分类号:

R382

曹胜魁, 姜岩岩, 袁忠英, 徐梦, 曹建平, 杨益超, 沈玉娟. 广西宾阳县和灵山县水源隐孢子虫和蓝氏贾第鞭毛虫污染调查[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(6): 597-601.

Sheng-kui CAO, Yan-yan JIANG, Zhong-ying YUAN, Meng XU, Jian-ping CAO, Yi-chao YANG, Yu-juan SHEN. Contamination of Cryptosporidium and Giardia lamblia in water from Binyang County and Lingshan County in Guangxi[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2018, 36(6): 597-601.

参考文献 29

[1]	Feng Y, Xiao L.Zoonotic potential and molecular epidemiology of Giardia species and giardiasis[J]. Clin Microbiol Rev, 2011, 24(1): 110-140.
[2]	Xiao L.Molecular epidemiology of cryptosporidiosis: an update[J]. Exp Parasitol, 2010, 124(1): 80-89.
[3]	Karanis P, Kourenti C, Smith H.Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt[J]. J Water Health, 2007, 5(1): 1-38.
[4]	Giangaspero A, Berrilli F, Brandonisio O.Giardia and Cryptosporidium and public health: the epidemiological scenario from the Italian perspective[J]. Parasitol Res, 2007, 101(5): 1169-1182.
[5]	Chalmers RM, Elwin K, Hadfield SJ, et al. Sporadic human cryptosporidiosis caused by Cryptosporidium cuniculus, United Kingdom, 2007-2008[J]. Emerging Infect Dis, 2011, 17(3): 536-538.
[6]	Feng Y, Wang L, Duan L, et al. Extended outbreak of cryptosporidiosis in a pediatric hospital, China[J]. Emerging Infect Dis, 2012, 18(2): 312-314.
[7]	Hoxie NJ, Davis JP, Vergeront JM, et al. Cryptosporidiosis-associated mortality following a massive waterborne outbreak in Milwaukee, Wisconsin[J]. Am J Public Health, 1997, 87(12): 2032-2035.
[8]	Florescu DF, Sandkovsky U.Cryptosporidium infection in solid organ transplantation[J]. World J Transplant, 2016, 6(3):460-471.
[9]	Liu H, Shen Y, Yin J, et al. Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in China[J]. BMC Infect Dis, 2014, 14(1): 25.
[10]	Wang T, Fan Y, Koehler AV, et al. First survey of Cryptosporidium, Giardia and Enterocytozoon in diarrhoeic children from Wuhan, China[J]. Infect Genet Evol, 2017, 51: 127-131.
[11]	Xu H, Jin Y, Wu W, et al. Genotypes of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in dogs and cats in Shanghai, China[J]. Parasit Vectors, 2016, 9: 121.
[12]	Efstratiou A, Ongerth J, Karanis P.Evolution of monitoring for Giardia and Cryptosporidium in water[J]. Water Res, 2017, 123: 96-112.
[13]	Feng Y, Zhao X, Chen J, et al. Occurrence, source, and human infection potential of Cryptosporidium and Giardia spp. in source and tap water in shanghai, China[J]. Appl Environ Microbiol, 2011, 77(11): 3609-3616.
[14]	Hashimoto A, Kunikane S, Hirata T.Prevalence of Cryptosporidium oocysts and Giardia cysts in the drinking water supply in Japan[J]. Water Res, 2002, 36(3): 519-526.
[15]	Kumar T, Add Majid MA, Onichandran S, et al. Presence of Cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system[J]. Infect Dis Poverty, 2016, 5: 3.
[16]	Zarlenga DS, Trout JM.Concentrating, purifying and detecting waterborne parasites[J]. Vet Parasitol, 2004, 126(1/2): 195-217.
[17]	Shepherd KM, Wynjones AP.An evaluation of methods for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water[J]. Appl Environ Microbiol, 1996, 62(4): 1317-1322.
[18]	张彤, 胡洪营, 宗祖胜. 再生水中隐孢子虫和贾第鞭毛虫的检测方法研究[J]. 中国给水排水, 2006, 22(5): 19-23, 29.
[19]	陈智敏, 张昱, 杨敏, 等. 密度梯度分离/免疫荧光技术检测再生水中隐孢子虫和贾第鞭毛虫[J]. 环境工程学报, 2011, 5(5): 982-986.
[20]	周美芝. 饮用水源水中“两虫”检测方法及其健康风险评价研究[D]. 杭州: 浙江工业大学, 2013.
[21]	Nieminski EC, Schaefer FW 3rd, Ongerth JE. Comparison of two methods for detection of Giardia cysts and Cryptosporidium oocysts in water[J]. Appl Environ Microbiol, 1995, 61(5): 1714-1719.
[22]	Vesey G, Slade JS, Byrne M, et al. A new method for the concentration of Cryptosporidium oocysts from water[J]. J Appl Bacteriol, 1993, 75(1): 82-86.
[23]	Xiao S, An W, Chen Z, et al. The burden of drinking water-associated cryptosporidiosis in China: the large contribution of the immunodeficient population identified by quantitative microbial risk assessment[J]. Water Res, 2012, 46(13): 4272-4280.
[24]	An W, Zhang D, Xiao S, et al. Risk assessment of Giardia in rivers of southern China based on continuous monitoring[J]. J Environ Sci (China), 2012, 24(2): 309-313.
[25]	Hu Y, Feng Y, Huang C, et al. Occurrence, source, and human infection potential of Cryptosporidium and Enterocytozoon bieneusi in drinking source water in Shanghai, China, during a pig carcass disposal incident[J]. Environ Sci Technol, 2014, 48(24): 14219-14227.
[26]	Xiao S, Hu S, Zhang Y, et al. Influence of sewage treatment plant effluent discharge into multipurpose river on its water quality: a quantitative health risk assessment of Cryptosporidium and Giardia[J]. Environ Pollut, 2018, 233: 797-805.
[27]	倪碧娴, 沈明学, 徐祥珍, 等. 江苏省生活饮用水中贾第鞭毛虫和隐孢子虫污染情况调查[J]. 中国血吸虫病防治杂志, 2017, 29(4): 454-458.
[28]	张小萍, 何艳燕, 朱倩, 等. 上海市饮用水和环境水中隐孢子虫和蓝氏贾第鞭毛虫污染状况调查[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(6): 435-438.
[29]	Ongerth JE, Saaed FM.Distribution of Cryptosporidium oocysts and Giardia cysts in water above and below the normal limit of detection[J]. Parasitol Res, 2013, 112(2): 467-471.