上海崇明发现稻富库蚊（双翅目∶蚊科）的记录

摘要/Abstract

摘要：

目的记录上海崇明发现稻富库蚊（Culex inatomii）。方法本研究于2015-2016年的5-11月在上海市崇明区东滩采集稻富库蚊的幼虫和成蚊,进行形态学观察。提取成蚊基因组DNA,PCR扩增线粒体细胞色素氧化酶亚基Ⅰ（COⅠ）基因片段。ClustalW2软件进行COⅠ序列多重比对。采用MEGA v5.10软件计算稻富库蚊与其同属的凶小库蚊（Cx. modestus）、淡色库蚊（Cx. pipiens pallens）和三带喙库蚊（Cx. tritaeniorhynchus）的种内和种间遗传距离。采用邻接法、最大似然法和贝叶斯法构建4个蚊种的系统进化树。结果共采集稻富库蚊成蚊156只,均为雌蚊;捕捞幼虫36只,实验室羽化后获雌蚊17只,雄蚊19只。形态学观察可见,雄蚊尾器结构中,抱肢基节亚端叶毛分为两组,前组具有2根棒状毛,后组具有1根棒状毛和1根叶状毛,可与其近缘种凶小库蚊区分。线粒体COⅠ基因片段PCR扩增获得约650 bp大小的条带,测序后提交GenBank（登录号为KX555565~KX555570）。COⅠ序列多重比对结果显示,稻富库蚊和凶小库蚊COⅠ序列的相似度为96%。两者种内遗传距离分别为0.001和0.011,种间遗传距离达0.047。系统进化树结果可见,4个蚊种聚成单系群,每个种独立为1个分支;稻富库蚊与凶小库蚊的亲缘关系最接近,而与同属其他2个蚊种的关系较远。结论上海崇明发现稻富库蚊。

关键词: 稻富库蚊, 凶小库蚊, 上海, 发现

Abstract:

Objective To record the discovery of Culex inatomii in Chongming, Shanghai. Methods Larvae and adult mosquitos of Cx. inatomii were collected in Dongtan of Chongming Island from May to November in 2015 and 2016, and their morphological characteristics were observed. The genomic DNA was extracted from adult mosquitos, PCR was performed to amplify the cytochrome c oxidaseⅠ(COⅠ) gene. Multiple alignment of COⅠ sequence was conducted with ClustalW2. Pairwise distances within and between species were calculated using MEGA v5.10 based on COⅠ sequences of Cx. inatomii, Cx. modestus, Cx. pipiens pallens, and Cx. tritaeniorhynchus. The phylogenetic tree of the above four species was constructed using neighbor joining, maximum likelihood, and Bayesian methods. Results One hundred and fifty-six adult female mosquitos and 36 larvae of Cx. inatomii were collected. Larvae were reared to adult stage in the laboratory (17 female, 19 male). Morphologically, the subapical lobe of the sidepiece in male genitalia was divided into two parts, the anterior part having 2 bladed setae, and the posterior part having 1 bladed setae and 1 lanceolar strong setae. This strucutre can be used to distinguish Cx. inatomii from Cx. modestus. PCR of COⅠ resulted in products of approximately 650 bp. They were sequenced and the sequencing result was submitted to GenBank (accession number, KX555565-KX555570). Multiple sequence alignment revealed a 96% sequence similarity of COⅠ between Cx. inatomii and Cx. modestus. The genetic distance between Cx. inatomii and Cx. modestus was 0.047, and that within them each was 0.003 and 0.011, respectively. The phylogenetic tree showed that the four species clustered as a monophyletic clade, and each formed an individual lineage. Cx. inatomii had a closer relationship with Cx. modestus, while distant from the other two species. Conclusion We recorded the discovery of Cx. inatomii in Chongming, Shanghai.

Key words: Culex inatomii, Culex modestus, Shanghai, Discovery

中图分类号:

R384.112

方圆, 施文琦, 张仪, 胡求安, 周正斌, 吴嘉彤, 张亮亮. 上海崇明发现稻富库蚊（双翅目∶蚊科）的记录[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(1): 31-35.

Yuan FANG, Wen-qi SHI, Yi ZHANG, Qiu-an HU, Zheng-bin ZHOU, Jia-tong WU, Liang-liang ZHANG. Discovery of Culex inatomii（Diptera ∶ Culicidae）in Chongming, Shanghai[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(1): 31-35.

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蚊种 Species	稻富库蚊 Cx. inatomii	三带喙库蚊 Cx. tritaeniorhynchus	淡色库蚊 Cx. pipiens pallens	凶小库蚊 Cx. modestus
稻富库蚊 Cx. inatomii	0.003
三带喙库蚊 Cx. tritaeniorhynchus	0.107	0.008
淡色库蚊 Cx. pipiens pallens	0.076	0.098	0.001
凶小库蚊 Cx. modestus	0.047	0.116	0.069	0.011