中国寄生虫学与寄生虫病杂志 ›› 2013, Vol. 31 ›› Issue (5): 10-376-381.

• 论著 • 上一篇    下一篇

广东珠三角地区胆囊结石患者的华支睾吸虫感染情况及其胆汁成分分析

罗小兵,乔铁*,马瑞红,郑培明,罗振亮,杨柳青,蔡洪英   

  1. 广州市南沙区第六人民医院,广州市番禺区胆囊病研究所,广州511470
  • 出版日期:2013-10-30 发布日期:2014-07-24

Survey on Clonorchis sinensis Infection and Bile Component of Gallstone Patients from the Pearl River Delta Region in Guangdong Province

LUO Xiao-bing, QIAO Tie*, MA Rui-hong, ZHENG Pei-ming, LUO Zhen-liang, YANG Liu-qing, CAI Hong-ying   

  1. The Sixth People′s Hospital of Nansha District, Guangzhou;Institute of Gallbladder Disease of Panyu District, Guangzhou,Guangzhou 511470, China
  • Online:2013-10-30 Published:2014-07-24

摘要: 目的  分析广东珠三角地区胆囊结石患者的华支睾吸虫感染情况和胆汁成分。  方法  采集2011年6月至2012年11月广州市南沙区第六人民医院普外科诊断的406例胆囊结石患者的粪便、血液、胆汁和胆囊结石标本。粪样采用改良加藤厚涂片法(Kato-Katz法)检查有无华支睾吸虫(Clonorchis sinensis)虫卵。血样采用胶体金免疫法检测华支睾吸虫抗体情况。胆汁离心后,取沉淀涂片,于显微镜下观察有无虫卵。胆囊结石采用研碎镜检法观察有无华支睾吸虫卵。取胆汁上清测定总钙(TCa)、离子钙(iCa)、HCO3-、Mg2+、pH、总胆红素(TBIL)、总胆汁酸(TBA)、总胆固醇(CHO)、谷氨酰转肽酶(GGT)和碱性磷酸酶(ALP)的含量。  结果  406例胆囊结石患者的华支睾吸虫感染率为52.2%(212/406),其中男性患者的华支睾吸虫感染阳性率为64.7%(130/201),高于女性患者(40.0%,82/205) (P<0.05)。胆汁上清检测结果显示,≤30岁的华支睾吸虫阳性患者胆汁中TBIL[(1 458.0±681.0)μmol/L]、CHO[(4.1±1.3)mmol/L]和Mg2+含量[(4.8±1.8)mmol/L],31~40岁阳性患者的TCa[(3.0±1.3)mmol/ L]、iCa[(1.5±0.7)mmol/L]、TBA[(114.6±54.5)mmol/L]、CHO[(5.1±1.7)mmol/L]、TBIL[(1 396.0±615.0)μmol/L]、GGT[(1 562.0±583.0)U/L]和ALP含量[(263.0±94.0)U/L]以及41~50岁阳性患者的CHO含量[(5.4±2.2)mmol/L]均低于对应阴性组[(2 759.0±969.0)μmol/L,(7.5±2.5)和(7.5±2.2)mmol/L;(3.8±1.6)、(1.9±1.0)、(144.1±63.4)和(9.9±2.5)mmol/L,(1 892.0±584.0)μmol/L,(2 457.0±988.0)和(535.0±196.0)U/L;(7.9±2.3)mmol/L](P<0.05);31~40岁阳性患者的HCO3-[(22.7±5.1)mmol/L]和pH(7.6±0.4)以及>50岁的pH(7.6±0.4)均高于对应阴性组[(17.3±6.9)mmol/L和7.4±0.2,7.5±0.3](P<0.05);其余各年龄段阳性患者的胆汁成分与阴性组的差异无统计学意义(P>0.05)。  结论  广东珠三角地区胆囊结石患者中华支睾吸虫感染率较高,且胆囊结石患者中,华支睾吸虫感染者与非感染者的胆汁成分有差别。

关键词: 胆囊结石, 华支睾吸虫, 胆汁成分

Abstract: Objective  To evaluate the prevalence of Clonorchis sinensis and bile components of gallstone patients from the Pearl River Delta region in Guangdong Province.  Methods  From June 2011 to November 2012, 406 gallstone patients from the Pearl River Delta region in Guangdong Province were enrolled in the study. Fecal specimens, bile and gallbladder stone samples were examined for the presence of C. sinensis eggs by the Kato-Katz method, centrifuged sediment smear microscopy, and triturated stone microscopy, respectively. The blood samples were detected for the antibody against C. sinensis by using colloidal gold immunochromatographic assay. Total calcium, ionized calcium, HCO3-, magnesium, pH, total bilirubin(TBIL), total bile acid(TBA), total cholesterol(CHO), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were analyzed quantitatively in bile of gall bladder.  Results  The overall C. sinensis infection rate was 52.2% (212/406). The proportion of males infected with C. sinensis was 64.7%(130/201), higher than that of females (40.0%, 82/205). The content of TBIL [(1 458.0±681.0) μmol/L], CHO [(4.1±1.3) mmol/L] and magnesium [(4.8±1.8) mmol/L] in the bile from the patients under 30 years old, tatal calcium [(3.0±1.3) mmol/L], ionized calcium[(1.5±0.7) mmol/L], TBA [(114.6±54.5) mmol/L], CHO [(5.1±1.7) mmol/L], TBIL [(1 396.0±615.0) μmol/L], GGT [(1 562.0±583.0) U/L] and ALP [(263.0±94.0) U/L] in 31-40 years-old, or CHO [(5.4±2.2) mmol/L] in 41-50 years-old in egg-positive group was lower than that of the corresponding negative groups [(2 759.0±969.0) μmol/L, (7.5±2.5), (7.5±2.2), (3.8±1.6), (1.9±1.0), (144.1±63.4), and (9.9±2.5) mmol/L, (1 892.0±584.0) μmol/L, (2 457.0±988.0) and (535.0±196.0) U/L, and (7.9±2.3) mmol/L], respectively (P<0.05). While, the content of HCO3- [(22.7±5.1) mmol/L], pH (7.6±0.4) in 31-40 years-old group and pH (7.6±0.4) in the patients aged above 50 years-old was higher than that of the negatives [(17.3±6.9) mmol/L, 7.4±0.2, and 7.5±0.3] (P<0.05).  Conclusion  In the Pearl River Delta region in Guangdong Province, C. sinensis prevalence is high in the gallstone patients. There are differences in bile components between C. sinensis-infected and non-infected patients.

Key words: Gallstone, Clonorchis sinensis, Bile component