中国寄生虫学与寄生虫病杂志 ›› 2011, Vol. 29 ›› Issue (5): 12-385-388.

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分子生物学技术在带绦虫鉴别中的应用

 李彦, 刘航, 杨毅梅   

  1. 大理学院基础医学院寄生虫教研室,大理 671000
  • 出版日期:2011-10-30 发布日期:2012-09-27

Application of Molecular Biological Techniques in Taenia Identification

 LI  Yan, LIU  Hang, YANG  Yi-Mei   

  1. Department of Parasitology,Dali University,Dali 671000,China
  • Online:2011-10-30 Published:2012-09-27

摘要: 统的带绦虫鉴别方法是根据成虫和其囊尾蚴的形态特征,但对于形态学特征相似的虫种鉴别较为困难。分子生物学技术提供了一种从分子水平来鉴别带绦虫的方法,使虫种鉴定结果更为客观。本文综述了DNA序列分析、PCR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。

关键词: 带绦虫, 分子生物学, 鉴别

Abstract: The traditional identification of Taenia spp. based on morphological features of adult and cysticercus has difficulties in identifying the morphologically similar species. The recent development of molecular techniques provides more scientific ways for distinguishing Taenia species. This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.

Key words: Taenia, Molecular biology, Identification