中国寄生虫学与寄生虫病杂志 ›› 2010, Vol. 28 ›› Issue (6): 8-435-438.

• 现场研究 • 上一篇    下一篇

上海市饮用水和环境水中隐孢子虫和蓝氏贾第鞭毛虫污染状况调查

张小萍,何艳燕,朱倩,马晓疆,蔡黎   

  1. 上海市疾病预防控制中心,上海 200336
  • 出版日期:2010-12-31 发布日期:2011-03-07

Investigation on Contamination of Cryptosporidium and Giardia in Drinking Water and Environmental Water in Shanghai

ZHANG Xiao-ping,HE Yan-yan,ZHU Qian,MA Xiao-jiang,CAI Li   

  1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336,China
  • Online:2010-12-31 Published:2011-03-07

摘要: 【摘要】  目的  了解上海市饮用水、水源水和环境水中隐孢子虫和蓝氏贾第鞭毛虫污染情况。 方法  通过抽滤、淘洗、离心浓缩、磁抗体分离和免疫荧光染色等技术检测饮用水、水源水和环境水。 结果  采集上海市16个区出厂水(32份)、 管网水(38份)和小区直饮水(86份)等水样156份,均未检出隐孢子虫卵囊和蓝氏贾第鞭毛虫包囊;采集5个区自来水厂水源水(15份)、 黄浦江原水(25份)、 动物饲养场周边河水(15份)、 污水处理厂出水(9份)和生活污水(6份)等水样70份, 隐孢子虫卵囊检出率分别为1份(6.7%)、 2份(8.0%)、 7份(46.7%)、 1份(11.1%)和1份(16.7%), 蓝氏贾第鞭毛虫包囊检出率分别为1份(6.7%)、 3份(12.0%)、 6份(40.0%)、 2份(22.2%)和2份(33.3%), 隐孢子虫卵囊总检出率为17.1%(12/70), 蓝氏贾第鞭毛虫包囊总检出率为20.0%(14/70)。 结论  上海市饮用水中未检出隐孢子虫卵囊和蓝氏贾第鞭毛虫包囊,但在水源水和环境水中检出,应加强自来水厂水源水的监测。

关键词: 隐孢子虫, 蓝氏贾第鞭毛虫, 饮用水, 水源水, 环境水, 污染

Abstract: 【Abstract】   Objective   To understand the contamination status of Cryptosporidium sp. and Giardia lamblia in drinking water, source water and environmental water in Shanghai.  Methods   All water samples collected from drinking water, source water and environmental water were detected by a procedure of micromembrane filtration, immune magnetic separation(IMS), and immunofluorescent assay(IFA).   Results   Cryptosporidium oocysts and Giardia cysts were not found in 156 samples of the drinking water including finished water, tap water, or pipe water for directly drinking in communities. Among 70 samples either source water of water plants(15 samples), environmental water from Huangpu River(25), canal water around animal sheds(15), exit water from waste-water treatment plants(9), or waste water due to daily life(6), Cryptosporidium oocysts were detected in 1(6.7%), 2(8.0%), 7(46.7%), 1(11.1%), and 1(16.7%) samples, respectively; and Giardia cysts were detected in 1(6.7%), 3(12.0%), 6 (40.0%), 2(22.2%), and 2(33.3%), respectively. The positive rate of  Cryptosporidium oocysts and Giardia cysts was 17.1%(12/70) and 20.0%(14/70), respectively.   Conclusion   No Cryptosporidium oocysts and Giardia cysts have been detected in drinking water, but found in source water and environmental water samples in Shanghai.

Key words: Cryptosporidium, Giardia lamblia, Drinking water, Source water, Environmental water, Contamination