关键词: 日本血吸虫, 乳酸脱氢酶, 生物信息学, 结构, 功能

Abstract: 【Abstract】 Objective To predict the structure and function of SjLDH using bioinformatics method. Methods By online analysis at bioinformatics websites such as NCBI（http：//www.ncbi.nlm.nih.gov/） and Expasy （http：//cn.expasy.org/）， and employing software packages such as Vector NTI suite and PCgene to do multi-sequence homological alignment，phylogenetic analysis， secondary structure and topological prediction， homology modeling of tertiary structure， antigenic epitope analysis， etc. Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH， TvLDH and HsLDH was 75%， 17%， 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH， the relationship between SjLDH and HsLDH-B， -C was closer than HsLDH-A. Three trans-membrane regions were found， the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （Pf.， Tg.， Tv.） and had 1-3 amino acid residue difference with MmLDH， HsLDH-A， -B， -C， and was the same with CsLDH. One of the key catalytic residues and substrate （pyruvate） binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop， other two key catalytic sites were at the position near the loop. Conclusion The prediction implied that LDH was an ideal molecule for phylogenetic analysis; SjLDH might be a potential molecular target for immunodiagnosis， anti-schistosome drug and vaccine development.

Key words: Schistosoma japonicum, Lactate dehydrogenase, Bioinformatics, Structure, Function