原位分子杂交检测上海真厉螨与柏氏禽刺螨体内肾综合症出血热病毒的研究

中国寄生虫学与寄生虫病杂志 ›› 1998, Vol. 16 ›› Issue (6): 441-444.

原位分子杂交检测上海真厉螨与柏氏禽刺螨体内肾综合症出血热病毒的研究

吴建伟¹; 孟阳春²; 李允鹤²; 周洪福²; 诸葛洪祥²; 赖培新³; 王建明³

1 遵义医学院寄生虫学教研室 2 苏州医学院寄生虫学教研室 3 贵州省遵义地区卫生防疫站

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-12-31 发布日期:1998-12-31

DETECTION OF HFRSV IN EULAELAPS SHANGHAIENSIS AND ORNITHONYSSUS BACOTI BY USING IN SITU HYBRIDIZATION

Wu Jianwei ^{1^△}; Meng Yangchun ²; Li Yunhe ²; Zhou Hongfu ²; Zhuge Hongxiang ²;
Lai Peixin ³; Wang Jianming ³

1 Department of Parasitology, Zunyi Medical College, Zunyi 563003 2 Department of Parasitology, Suzhou Medical College, Suzhou　2150073 Zunyi Health and Prevention Station of Diseases, Zunyi　563000

Received:1900-01-01 Revised:1900-01-01 Online:1998-12-31 Published:1998-12-31

摘要/Abstract

摘要： 目的：提供革螨传播肾综合征出血热病毒（HFRSV）的分子生物学证据。方法：用上海真厉螨与柏氏禽刺螨叮咬HFRSV接种乳小鼠，取叮咬后d10及d100以上的上海真厉螨与柏氏禽刺螨冰冻切片，用地高辛标记HFRSVcDNA核酸探针原位分子杂交检测其体内病毒RNA。结果：叮咬野鼠型（76－118株）接种乳小鼠后d10上海真厉螨检出病毒RNA，在细胞浆、细胞核中及核膜上呈细密颗粒状或全浆阳性，见于生殖器官细胞、胃及支囊上皮细胞及脑皮质细胞；叮咬家鼠型（UR株）病毒接种乳小鼠后d12螨亦检出病毒RNA，病毒的细胞组织分布与野鼠型相同；分别检测野鼠型螨31 只和家鼠型23 只, 其中阳性分别为17 只和10 只; 叮咬野鼠型病毒接种乳小鼠后d132, 家鼠型d102螨仍能检出病毒RNA。柏氏禽刺螨叮咬野鼠型病毒接种乳小鼠d10, 检测螨20只, 其中阳性12 只, 病毒RNA 主要分布于生殖器官细胞内。结论: 上海真厉螨与柏氏禽刺螨均可经叮咬获得HFRSV , 上海真厉螨可分别感染野鼠型和家鼠型HFRSV , 野鼠型在螨群体内至少可维持132 d, 家鼠型102 d, 具有贮存两型病毒的作用, 是HFRSV 的适宜媒介和贮存宿主, 在动物宿主间交叉传播两型HFRSV 中起重要作用。

关键词: 革螨, 上海真厉螨, 柏氏禽刺螨, 原位分子杂交, 肾综合征出血热病毒RNA

Abstract: AIM:To provide molecular biological evidence of transmission of hemorrhogic fever
with renal syndrome virus (HFRSV) by gamasid mites, Eulaelaps shanghaiensis and Ornithonyssus
bacoti .METHODS: Frozen sections of the gamasid mites 10 days and more than 100 days after
biting suckling mice inoculated with HFRSV were in situ hybridized with dig labelled HFRSV cDNA
probes .RESULTS: RNA was detected in frozen sections of Eulaelaps shanghaiensis , after biting
suckling mice inoculated with Hantaan (76-118) and Seoul (UR ) virus, respectively. Most of the fine granules of the virus RNA were located in the nuclei, cytoplasm and nuclear membrane of cells of brain cortex, caeca and genitalia of the mites. In situ hybridization results showed that 17 of 31 mites in Hantaan group and 10 of 23 mites in Seoul group were positive. The virus RNA was still detected in tissues of the mites on d132 for Hantaan group and on d102 for Seoul group after infection, respectively. Among 20 Ornithonyssus bacoti detected 12 were positive on d10 after biting suckling mice inoculated with Hantaan virus, and the virus RNA was mainly found in the cells of genitalia (Figs. 1- 13). CONCLUSION: Both Eulaelaps shanghaiensis and Ornithonyssus bacoti could be infected with HFRSV by biting HFRSV-positive mice . E. shanghaiensis could be infected with both Hantaan and Seoul virus, and the two types of HFRSV were found to be maintained in the mites for 132 days and 102 days, respectively. These confirmed that E. shanghaiensis and O. bacotiare suitable vectors and reservoirs of both Hantaan and Seoul virus and might play an important role in the cross transmission of the two types of HFRSV.

Key words: Gamasid mites, Eulaelaps shanghaiensis, Ornithonyssus bacoti, In situ hybridization, HFRSV RNA

吴建伟;孟阳春;李允鹤;周洪福;诸葛洪祥;赖培新;王建明. 原位分子杂交检测上海真厉螨与柏氏禽刺螨体内肾综合症出血热病毒的研究[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(6): 441-444.

WuJianwei△;MengYangchun;LiYunhe;ZhouHongfu;ZhugeHongxiang;LaiPeixin;WangJianming. DETECTION OF HFRSV IN EULAELAPS SHANGHAIENSIS AND ORNITHONYSSUS BACOTI BY USING IN SITU HYBRIDIZATION[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1998, 16(6): 441-444.

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