云南大理毛腿鼠耳蝠体表寄生虫感染情况及其体表寄生蛛蝇的形态特征和系统进化分析

doi:10.12140/j.issn.1000-7423.2023.04.009

中国寄生虫学与寄生虫病杂志 ›› 2023, Vol. 41 ›› Issue (4): 452-458.doi: 10.12140/j.issn.1000-7423.2023.04.009

云南大理毛腿鼠耳蝠体表寄生虫感染情况及其体表寄生蛛蝇的形态特征和系统进化分析

杨金颋¹(), 黄晓宾¹^,^*(), 王玉娟², 郭宪国¹, 张现政¹, 杨慧娟¹, 郑小燕¹

1 大理大学病原与媒介生物研究所，云南省自然疫源性疾病防控技术重点实验室，云南大理 671000
2 东北师范大学吉林省动物资源保护与利用重点实验室，吉林长春 130000

收稿日期:2022-12-31 修回日期:2023-04-20 出版日期:2023-08-30 发布日期:2023-09-06
通讯作者: *黄晓宾（1989-），男，博士，助理研究员，从事媒介生物学研究。E-mail：huangxb633@nenu.edu.cn
作者简介:杨金颋（1997-），男，硕士研究生，从事病原生物学研究。E-mail：yangjinting97@163.com
基金资助:
国家自然科学基金(32001096);云南省科技厅科技计划(202001AT070025)

Myotis fimbriatus ectoparasite infection and the morphological and phylogenetic analysis of Nycteribiidae in Dali, Yunnan Province

YANG Jinting¹(), HUANG Xiaobin¹^,^*(), WANG Yujuan², GUO Xianguo¹, ZHANG Xianzheng¹, YANG Huijuan¹, ZHENG Xiaoyan¹

1 Institute of Pathogens and Vectors, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali University, Dali 671000, Yunnan, China
2 Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun 130000, Jilin, China

Received:2022-12-31 Revised:2023-04-20 Online:2023-08-30 Published:2023-09-06
Contact: *E-mail: huangxb633@nenu.edu.cn
Supported by:
National Natural Science Foundation of China(32001096);Yunnan Provincial Science and Technology DepartmentScience and Technology Plan(202001AT070025)

摘要/Abstract

摘要：

目的对云南大理毛腿鼠耳蝠体表寄生虫的感染状况进行分析，并描述两种蛛蝇的形态特征，探究其系统进化关系。方法 2022年7月，在云南大理州剑川县捕捉毛腿鼠耳蝠，采集其体表寄生虫并进行鉴定。计算各体表寄生虫的构成比、感染率、平均多度和平均感染强度；使用优势度指数（Y）确定各体表寄生虫类群中的优势种；运用扩散指数（C）、I指数、聚块指数（m*/m）和K指数判断优势种的空间分布类型；利用协调系数（V）分析优势种之间的相互关系。描述两种寄生蛛蝇的主要形态学特征。提取蛛蝇基因组DNA，PCR扩增线粒体细胞色素C氧化酶1亚基（cox1）、细胞色素B（cytb）和16S核糖体RNA（16S rRNA）序列，测序并串联后，用MEGA 11以最大似然法构建基于这3个基因的系统进化树。结果共捕获毛腿鼠耳蝠72只（雄性24只，雌性48只），体表寄生虫感染率为97.3%（70/72）。共采集体表寄生虫1 745只，其中革螨1 463只，分属于2科5属7种，分别为织金巨刺螨、鼠耳蝠螨、围睫肪刺螨、柯氏蝠螨、盾板浆刺螨、宽埃螨和朝鲜巨刺螨；蛛蝇282只，分属于2属2种，分别为台湾蛛虱蝇和叉笔虱蝇；未检获其他体表寄生虫。毛腿鼠耳蝠的革螨感染率、平均多度和平均感染强度分别为94.4%（68/72）、20.32、21.53，均高于蛛蝇[91.7%（66/72）、3.92、4.27]（Fisher精确检验，P < 0.05；U = 700、424，均P < 0.01）。织金巨刺螨、鼠耳蝠螨和围睫肪刺螨为优势种（Y = 0.30、0.23、0.22）。优势种鼠耳蝠螨（C = 1.98、I = 0.98、m*/m = 1.18、K = 5.55）、织金巨刺螨（C = 9.43、I = 8.43、m*/m = 2.15、K = 0.87）和围睫肪刺螨（C = 6.50、I = 5.50、m*/m = 2.02、K = 0.98）在不同毛腿鼠耳蝠个体间均呈现聚集分布。鼠耳蝠螨与围睫肪刺螨（V = 0.33，P < 0.05）、鼠耳蝠螨与织金巨刺螨（V = 0.34，P < 0.05）、围睫肪刺螨与织金巨刺螨（V = 0.72，P < 0.01）之间均存在正协调关系。台湾蛛虱蝇体长约3 mm，背板Ⅰ呈宽圆形，末端有约25根长刚毛，背板Ⅱ和背板Ⅵ之间有较多长毛，背板Ⅵ上有6根长刚毛和10根长刺。叉笔虱蝇体长为3.5~4.0 mm，背板Ⅰ呈长宽状，边缘有一排短刚毛，背板Ⅱ长约为宽的3倍，背板Ⅱ后方有两个突起，突起表面呈凹状，后缘尖锐带有色素沉着。台湾蛛虱蝇cox1、cytb和16S rRNA序列分别为875、338和455 bp，提交至GenBank获得的登录号分别为OQ127648、OQ134944和OQ127330；叉笔虱蝇cox1、cytb和16S rRNA序列分别为832、332和451 bp，提交至GenBank获得的登录号分别为OQ119629、OQ129602和OQ119632。系统进化树分析结果显示，台湾蛛虱蝇与短铗蛛虱蝇聚在同一个分支上，再与叉笔虱蝇聚在一支。结论云南大理毛腿鼠耳蝠体表寄生虫感染率高，主要为革螨和蛛蝇，其中台湾蛛虱蝇与同属的短铗蛛虱蝇的亲缘关系最近，叉笔虱蝇与台湾蛛虱蝇和铗蛛虱蝇的亲缘关系较近。

关键词: 毛腿鼠耳蝠, 体表寄生虫, 云南大理, 革螨, 蛛蝇

Abstract:

Objective To analyze the prevalence of ectoparasites on the surface of Myotis fimbriatus in Dali, Yunnan Province, and describe the morphological characteristics of two Nycteribiidae, exploring their phylogenetic relationship. Methods In July 2022, M. fimbriatus were captured in Jianchuan County, Dali Prefecture, Yunnan Province. The ectoparasites were collected and identified. The constituent ratio, infection rate, mean abundance, and mean intensity were calculated for each ectoparasite. Dominant species were determined using the dominance index (Y). Their spatial distribution types were determined using the dispersion coefficient (C), I index, patchiness index (m*/m), and K index. The association coefficient (V) was used to analyze the interaction between dominant species. The morphological characteristics of the two Nycteribiidae were described. Genomic DNA was extracted from Nycteribiidae, and the mitochondrial cytochrome C oxidase subunit 1 (cox1), cytochrome B (cytb) and 16S ribosomal RNA (16S rRNA) was amplified by PCR and sequenced, then the sequences in tandem were used to construct the phylogenetic tree using the maximum likelihood method in MEGA 11. Results A total of 72 M. fimbriatus (24 males, 48 females) were captured, and the ectoparasites infection rate was 97.3% (70/72). A total of 1 745 ectoparasites were collected, including 1 463 gamasid mites belonging to 7 species in 5 genera and 2 families, namely Spinturnix myoti, Spinturnix kolenatii, Steatonyssus periblepharus, Macronyssus zhijinensis, Choronyssus scutatus, Eyndhovenia euryalis, and Macronyssus coreanus. Additionally, 282 Nycteribiidae were collected, which belongs to 2 species in 2 genera, namely Nycteribia formosana and Penicillidia dufourii. No other ectoparasites were detected. The infection rate, mean abundance, and mean intensity of gamasid mites are 94.4% (68/72), 20.32 and 21.53, respectively, all of which are higher than those of the Nycteribiidae [91.7% (66/72), 3.92, 4.27] (Fisher's exact test, P < 0.05; U = 700, 424; both P < 0.01). S. myoti, S. periblepharus and M. zhijinensis are the dominant species (Y = 0.30, 0.23, 0.22). The dominant species, including S. myoti (C = 1.98, I = 0.98, m*/m = 1.18, K = 5.55), M. zhijinensis (C = 9.43, I = 8.43, m*/m = 2.15, K = 0.87) and S. periblepharus (C = 6.50, I = 5.50, m*/m = 2.02, K = 0.98), exhibit an aggregated distribution among different individuals of M. fimbriatus. There is a positive correlation relationship between S. myoti and S. periblepharus (V = 0.33, P < 0.05), between S. myoti and M. zhijinensis (V = 0.34, P < 0.05), and between S. periblepharus and M. zhijinensis (V = 0.72, P < 0.01). N. formosana has a body length of about 3 mm. Its dorsal plate Ⅰ is wide and round, with about 25 long bristles at the tip. There are many long hairs between the dorsal plates Ⅱ and Ⅵ, and the dorsal plate Ⅵ has 6 long bristles and 10 long spikes. P. dufourii has a body length of 3.5-4.0 mm. The dorsal plate Ⅰ is elongated and wide, with a row of short bristles along the edges. The dorsal plate Ⅱ is approximately three times longer than it is width. At the back of the dorsal plate Ⅱ, there are two protrusions with concave surfaces. The posterior edge of the protrusions is sharp and has pigmented deposits. The cox1, cytb and 16S rRNA sequences of N. formosana were 875, 338 and 455 bp, respectively. The GenBank accession numbers are OQ127648, OQ134944, and OQ127330. The cox1, cytb and 16S rRNA sequences of P. dufourii were 832, 332 and 451 bp, respectively. The GenBank accession numbers are OQ119629, OQ129602, and OQ119632. The results of the phylogenetic tree analysis show that N. formosana and the N. parvula are clustered together in the same branch, and then they are clustered with P. dufourii. Conclusion The infection rate of ectoparasites on M. fimbriatus in Yunnan Dali is high, mainly with the presence of gamasid mites and Nycteribiidae, among them, N. formosana shows the closest genetic relationship to its congeneric N. parvula, while P. dufourii is related closer to N. formosana and N. parvula.

Key words: Myotis fimbriatus, Ectoparasites, Dali, Yunnan, Gamasid mites, Nycteribiidae

中图分类号:

R384

杨金颋, 黄晓宾, 王玉娟, 郭宪国, 张现政, 杨慧娟, 郑小燕. 云南大理毛腿鼠耳蝠体表寄生虫感染情况及其体表寄生蛛蝇的形态特征和系统进化分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 452-458.

YANG Jinting, HUANG Xiaobin, WANG Yujuan, GUO Xianguo, ZHANG Xianzheng, YANG Huijuan, ZHENG Xiaoyan. Myotis fimbriatus ectoparasite infection and the morphological and phylogenetic analysis of Nycteribiidae in Dali, Yunnan Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2023, 41(4): 452-458.

图/表 4

表1

表2

图1

图2

参考文献 34

[1]	Fenton MB, Mubareka S, Tsang SM, et al. COVID-19 and threats to bats[J]. FACETS, 2020, 5(1): 349-352. doi: 10.1139/facets-2020-0028
[2]	Fan Y, Zhao K, Shi ZL, et al. Bat coronaviruses in China[J]. Viruses, 2019, 11(3): 210. doi: 10.3390/v11030210
[3]	Fan BZ, Huang XB, Guo XG, et al. Ectoparasites on body surface of Rhinolophus sinicus in Yunnan[J]. Chin Trop Med, 2022, 22(3): 229-233. (in Chinese)
	(范宝珠, 黄晓宾, 郭宪国, 等. 云南省中华菊头蝠体表寄生虫感染状况[J]. 中国热带医学, 2022, 22(3): 229-233.) doi: 10.13604/j.cnki.46-1064/r.2022.03.07
[4]	Chen HX, Qiu FX, Dong BJ, et al. Epidemiological studies on hemorrhagic fever with renal syndrome in China[J]. J Infect Dis, 1986, 154(3): 394-398. pmid: 2874178
[5]	Barbieri R, Signoli M, Chevé D, et al. Yersinia pestis: the natural history of plague[J]. Clin Microbiol Rev, 2020, 34(1): e00044-19.
[6]	Chung MH, Kang JS. History of tsutsugamushi disease in Korea[J]. Infect Chemother, 2019, 51(2): 196-209. doi: 10.3947/ic.2019.51.2.196 pmid: 31271001
[7]	Han HJ, Li ZM, Li X, et al. Bats and their ectoparasites (Nycteribiidae and Spinturnicidae) carry diverse novel Bartonella genotypes, China[J]. Transbound Emerg Dis, 2022, 69(4): e845-e858.
[8]	Banerjee A, Baid K, Byron T, et al. Seroprevalence in bats and detection of Borrelia burgdorferi in bat ectoparasites[J]. Microorganisms, 2020, 8(3): 440. doi: 10.3390/microorganisms8030440
[9]	Szentiványi T, Christe P, Glaizot O. Bat flies and their microparasites: current knowledge and distribution[J]. Front Vet Sci, 2019, 6: 115. doi: 10.3389/fvets.2019.00115 pmid: 31106212
[10]	Liu Y, Feng J, Chen M, et al. The analysis on echolocation calls of Myotis fimbriatus (Chiroptera :Vespertilionidae)[J]. J Northeast Norm Univ Nat Sci Ed, 2003, 35(2): 113-116. (in Chinese)
	(刘颖, 冯江, 陈敏, 等. 毛腿鼠耳蝠回声定位声波的分析[J]. 东北师大学报(自然科学版), 2003, 35(2): 113-116.)
[11]	Armero A, Li RY, Bienes KM, et al. Myotis fimbriatus virome, a window to virus diversity and evolution in the genus Myotis[J]. Viruses, 2022, 14(9): 1899. doi: 10.3390/v14091899
[12]	Pan QH, Wang YX, Yan K. A field guide to the mammals of China[M]. Beijing: China Forestry Publishing House, 2007: 41-76. (in Chinese)
	(潘清华, 王应祥, 岩崑. 中国哺乳动物彩色图鉴[M]. 北京: 中国林业出版社, 2007: 41-76.)
[13]	Pan GF, Dun Q, Gu YM. Economic insect fauna of China:fasc. 40, Acari: Dermanyssoideae[M]. Beijing: Science Press, 1993: 301-366.
	(邓国藩, 王敦清, 顾以铭. 中国经济昆虫志: 第四十册蜱螨亚纲蜱刺螨总科[M]. 北京: 科学出版社, 1993: 301-366.)
[14]	Miriam R, Oskar T. Illustrated catalogue of the Rothschild collection of Nycteribiidae (Diptera) in the British Museum (Natural History)[M]. London: British Museum (Natural History), 1967: 43-46.
[15]	Chen YL, Guo XG, Ren TG, et al. Ecological analysis of mites on the body surface of Rattus brunneusculus in Yunnan Province from 2001 to 2015[J]. Chin J Dis Control Prev, 2021, 25(12): 1470-1475. (in Chinese)
	(陈雁翎, 郭宪国, 任天广, 等. 云南省2001―2015年东亚屋顶鼠体表螨类生态学分析[J]. 中华疾病控制杂志, 2021, 25(12): 1470-1475.)
[16]	Zhang C, Ge X, Zhao L, et al. The spatial distribution pattern of overwintering egg of Monolepta hieroglyphica (Motschulsky) in corn field[J]. Acta Ecol Sin, 2013, 33(11): 3452-3459. (inChinese)
	(张聪, 葛星, 赵磊, 等. 双斑长跗萤叶甲越冬卵在玉米田的空间分布型[J]. 生态学报, 2013, 33(11): 3452-3459.)
[17]	Liu Z, Guo XG, Fan R, et al. Ecological analysis of gamasid mites on the body surface of Norway rats (Rattus norvegicus) in Yunnan Province, Southwest China[J]. Biologia, 2020, 75(9): 1325-1336. doi: 10.2478/s11756-019-00383-z
[18]	Postawa T, Furman A. Abundance patterns of ectoparasites infesting different populations of Miniopterus species in their contact zone in Asia minor[J]. Acta Chiropterologica, 2014, 16(2): 387-395. doi: 10.3161/150811014X687341
[19]	Gibson JF, Kelso S, Jackson MD, et al. Diptera-specific polymerase chain reaction amplification primers of use in molecular phylogenetic research[J]. Ann Entomol Soc Am, 2011, 104(5): 976-997. doi: 10.1603/AN10153
[20]	Tamura K, Stecher G, PetersonD, et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol, 2013, 30(12): 2725-2729. doi: 10.1093/molbev/mst197 pmid: 24132122
[21]	Burazerovic J, Orlova M, Obradovic M, et al. Patterns of abundance and host specificity of bat ectoparasites in the central Balkans[J]. J Med Entomol, 2018, 55(1): 20-28. doi: 10.1093/jme/tjx189 pmid: 29029286
[22]	Ding F, Liu XH, Guo XG, et al. Analysis of gamasidmites(Acari : Mesostigmata) on Mus pahari in Yunnan Province[J]. Chin J Zool, 2021, 56(2): 240-246. (in Chinese)
	(丁帆, 刘昕航, 郭宪国, 等. 云南省锡金小鼠体表革螨感染分析[J]. 动物学杂志, 2021, 56(2): 240-246.)
[23]	Li B, Guo XG, Ren TG, et al. Infestation and related ecological analyses of mites on mole shrews (Anourosorex squamipes) in Yunnan Province[J]. Chin J Zool, 2021, 56(5): 686-695. (in Chinese)
	(李贝, 郭宪国, 任天广, 等. 云南省短尾鼩体表螨类感染及相关生态分析[J]. 动物学杂志, 2021, 56(5): 686-695.)
[24]	Orlova MV, Orlov OL, ZhigalinAV. New records of ectoparasites of the eastern water bat Myotis petax Hollister, 1912 (Vespertilionidae, Chiroptera) and the revision of the previousmaterial collected from Myotis daubentonii s. lato in Eastern Palaearctic[J]. Parazitologiia, 2014, 48(4): 315-324. pmid: 25702504
[25]	Bruyndonckx N, Dubey S, Ruedi M, et al. Molecular cophylogenetic relationships between European bats and their ectoparasitic mites (Acari, Spinturnicidae)[J]. Mol Phylogenetics Evol, 2009, 51(2): 227-237. doi: 10.1016/j.ympev.2009.02.005
[26]	Frank R, Kuhn T, Werblow A, et al. Parasite diversity of European Myotis species with special emphasis on Myotis myotis (Microchiroptera, Vespertilionidae) from a typical nursery roost[J]. Parasites Vectors, 2015, 8(1): 101. doi: 10.1186/s13071-015-0707-7
[27]	Orlova MV, Zapart A. Interaction of ectoparasites in cohabitating colonies of pond bats Myotis dasycneme (Boie, 1825) and species of genus Pipistrellus from northern Poland[J]. Ann Parasitol, 2012, 58(4): 211-215. pmid: 23914616
[28]	Cicek H, Stanyukovich M, Yağci S, et al. Gamasine mite (Parasitiformes : Mesostigmata) infestations of small mammals (Mammalia : Rodentia, Insectivora) in Turkey[J]. Turkiye Parazitol Derg, 2008, 32(1): 65-70.
[29]	Zabashta MV, Orlova MV, Pichurina NL, et al. Participation of bats (Chiroptera, Mammalia) and their ectoparasites in circulation of pathogens of natural focal infections in the south of Russia[J]. Entmol Rev, 2019, 99(4): 513-521. doi: 10.1134/S0013873819040110
[30]	Yang HJ, Yang ZH, Chen T, et al. A comparative study of mitochondrial protein-coding genes in the Gamasida[J]. J Pathog Biol, 2022, 17(11): 1303-1308, 1314.(in Chinese)
	(杨慧娟, 杨志华, 陈婷, 等. 革螨亚目线粒体蛋白编码基因的比较研究[J]. 中国病原生物学杂志, 2022, 17(11):1303-1308, 1314.)
[31]	Lučan RK. Relationships between the parasitic mite Spinturnix andegavinus (Acari : Spinturnicidae) and its bat host, Myotis daubentonii (Chiroptera : Vespertilionidae): seasonal, sex- and age-related variation in infestation and possible impact of the parasite on the host condition and roosting behaviour[J]. Folia Parasit, 2006, 53(2): 147-152. doi: 10.14411/fp.2006.019
[32]	Czenze ZJ, Broders HG. Ectoparasite community structure of two bats (Myotis lucifugus and M. septentrionalis) from the Maritimes of Canada[J]. J Parasitol Res, 2011, 2011: 341535.
[33]	Zahn A, Rupp D. Ectoparasite load in European vespertilionid bats[J]. J Zool, 2004, 262(4): 383-391. doi: 10.1017/S0952836903004722
[34]	Moura MO, Bordignon MO, Graciolli G. Host characteristics do not affect community structure of ectoparasites on the fishing bat Noctilio leporinus (L., 1758) (Mammalia : Chiroptera)[J]. Mem Inst Oswaldo Cruz, 2003, 98(6): 811-815. doi: 10.1590/S0074-02762003000600017

体表寄生虫种类 Species of ectoparasite	体表寄生虫数 No. ectoparasite	体表寄生虫构成比/% Constituent ratio of ectoparasite/%	蝙蝠感染数 No. bat infected	蝙蝠感染率/% Infection rate of bat/%	平均多度 Mean abundance	平均感染强度 Mean intensity
革螨 Gamasid mite
鼠耳蝠螨 Spinturnix myoti	393	22.5（393/1 745）	68	94.4（68/72）	5.46	5.78
柯氏蝠螨 Spinturnix kolenatii	137	7.8（137/1 745）	56	77.8（56/72）	1.90	2.45
围睫肪刺螨 Steatonyssus periblepharus	387	22.2（387/1 745）	47	65.3（47/72）	5.38	8.23
织金巨刺螨 Macronyssus zhijinensis	529	30.3（529/1 745）	48	66.7（48/72）	7.35	11.02
盾板浆刺螨 Ichoronyssus scutatus	15	0.8（15/1 745）	8	11.1（8/72）	0.21	1.88
宽埃螨 Eyndhovenia euryalis	1	0.1（1/1 745）	1	1.4（1/72）	0.01	1.00
朝鲜巨刺螨 Macronyssus coreanus	1	0.1（1/1 745）	1	1.4（1/72）	0.01	1.00
小计 Subtotal	1 463	83.8（1 463/1 745）	68	94.4（68/72）	20.32	21.52
蛛蝇 Nycteribiidae
台湾蛛虱蝇 Nycteribia formosana	186	10.7（186/1 745）	56	77.8（56/72）	2.58	3.32
叉笔虱蝇 Penicillidia dufourii	96	5.5（96/1 745）	49	68.1（49/72）	1.33	1.96
小计 Subtotal	282	16.2（282/1 745）	66	91.7（66/72）	3.92	4.27
合计 Total	1 745	100（1 745/1 745)	70	97.2（70/72）	24.24	24.93

性别 Gender	感染率/% Prevalence/%			平均多度 Mean abundance			平均感染强度 Mean intensity
性别 Gender	蛛蝇 Nycteribiidae	革螨 Gamasid mite	总体表寄生虫 Total ectoparasites	蛛蝇 Nycteribiidae	革螨 Gamasid mite	总体表寄生虫 Total ectoparasites	蛛蝇 Nycteribiidae	革螨 Gamasid mite	总体表寄生虫 Total ectoparasites
雄性 Male	83.3（60/72）	91.7（66/72）	91.7（66/72）	3.38	19.50	22.92	4.05	21.27	25.00
雌性 Female	95.8（69/72）	95.8（69/72）	100（72/72）	4.19	19.90	24.94	4.47	22.11	24.94
χ²/U	3.27	0.53		438.5	554.5	352.5	394.5	503.5	523.5
P	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05

云南大理毛腿鼠耳蝠体表寄生虫感染情况及其体表寄生蛛蝇的形态特征和系统进化分析

Myotis fimbriatus ectoparasite infection and the morphological and phylogenetic analysis of Nycteribiidae in Dali, Yunnan Province

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 34

相关文章 7

编辑推荐

Metrics

本文评价

[1]	董文鸽;郭宪国;门兴元;钱体军;吴滇. 洱海周边地带社鼠体表寄生虫的多样性调查[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(3): 12-244.
[2]	刘耕;关国华;王彦玲;姚文丽;朴昌吉. 牡丹江市一次革螨所致皮炎的暴发流行[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(5): 21-267.
[3]	吴建伟;孟阳春;李允鹤;周洪福;诸葛洪祥;赖培新;王建明. 原位分子杂交检测上海真厉螨与柏氏禽刺螨体内肾综合症出血热病毒的研究[J]. 中国寄生虫学与寄生虫病杂志, 1998, 16(6): 441-444.
[4]	郭宪国, 叶炳辉, 顾以铭, 陈渊民. 云南西部小兽革螨群落相似性及分类研究[J]. 中国寄生虫学与寄生虫病杂志, 1996, 14(1): 42-45.
[5]	林金学. 革螨体外寄生一例报道[J]. 中国寄生虫学与寄生虫病杂志, 1993, 11(3): 218-218.
[6]	张悟澄,周全春,曾庆仁,吴纯. 湖北肋壳钉螺与云南大理光壳钉螺逸出的尾蚴的致病力实验研究[J]. 中国寄生虫学与寄生虫病杂志, 1988, 6(S1): 48-48.
[7]	顾以铭,王菊生. 贵州省的革螨[J]. 中国寄生虫学与寄生虫病杂志, 1988, 6(S1): 144-144.